BRENDA - Enzyme Database

Purification and characterization of membrane-bound and cytosolic forms of diacylglycerol kinase from rat brain

Kato, M.; Takenawa, T.; J. Biol. Chem. 265, 794-800 (1990)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.107
detergent
no activity in absence of detergent
Rattus norvegicus
2.7.1.107
phosphatidic acid
good activator of DGK IV, no effect on DGK I activity
Rattus norvegicus
2.7.1.107
phosphatidylcholine
moderate enhancement of DGK IV, no effect on DGK I activity
Rattus norvegicus
2.7.1.107
phosphatidylinositol
enhances activity of DGK I and DGK IV
Rattus norvegicus
2.7.1.107
phosphatidylserine
enhances activity of DGK I and DGK IV
Rattus norvegicus
2.7.1.107
sodium cholate
enhances activity of DGK I and DGK IV
Rattus norvegicus
2.7.1.107
sodium deoxycholate
enhances activity of DGK I and DGK IV
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.107
adenosine
0.5 mM, 5% inhibition of DGK I and 28% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
ADP
0.5 mM, 77% inhibition of DGK I and 66% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
AMP
0.5 mM, 18% inhibition of DGK I and 14% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
ATPgammaS
0.5 mM, 93% inhibition of DGK I and 71% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
cAMP
0.5 mM, 20% inhibition of DGK I and 15% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
CDP
0.5 mM, 43% inhibition of DGK I and 28% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
CTP
0.5 mM, 42% inhibition of DGK I and 9% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
GDP
0.5 mM, 74% inhibition of DGK I and 72% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
GTP
0.5 mM, 74% inhibition of DGK I and 56% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
GTPgammaS
0.5 mM, 37% inhibition of DGK I and 32% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
quercetin
0.1 mM, 41% inhibition of DGK I and 15% inhibition of DGK IV
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.107
0.07
-
1-stearoyl-2-arachidonoyl-glycerol
pH 7.4, 30C, enzyme form DGK I
Rattus norvegicus
2.7.1.107
0.09
-
1,2-diarachidonoyl-glycerol
pH 7.4, 30C, enzyme form DGK I
Rattus norvegicus
2.7.1.107
0.09
-
1-stearoyl-2-arachidonoyl-glycerol
pH 7.4, 30C, enzyme form DGK IV
Rattus norvegicus
2.7.1.107
0.1
-
sn-1,2-dioleoylglycerol
pH 7.4, 30C, enzyme form DGK IV
Rattus norvegicus
2.7.1.107
0.125
-
ATP
pH 7.4, 30C, in presence of 1,2-dioleoylglycerol, enzyme form DGK I
Rattus norvegicus
2.7.1.107
0.125
-
sn-1,2-dioleoylglycerol
pH 7.4, 30C, enzyme form DGK I
Rattus norvegicus
2.7.1.107
0.14
-
1,2-diarachidonoyl-glycerol
pH 7.4, 30C, enzyme form DGK IV
Rattus norvegicus
2.7.1.107
0.25
-
ATP
pH 7.4, 30C, in presence of 1,2-dioleoylglycerol, enzyme form DGK IV
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.107
cytosol
DGK I
Rattus norvegicus
5829
-
2.7.1.107
membrane
bound to; DGK IV
Rattus norvegicus
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.107
Ca2+
DGK I: good activator, DGK IV: activation with 1 mM CaCl2 is comparable to that obtained on presence of MgCl2
Rattus norvegicus
2.7.1.107
Mg2+
DGK I: maximal activation at 20 mM. DGK IV: maximal activation at 5 mM
Rattus norvegicus
2.7.1.107
Mn2+
DGK I: activation is not so great as with MgCl2. Maximal stimulation at 0.1 mM. DGK IV: activation is 10fold less than with MgCl2
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.107
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.107
DGK I and DGK IV
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.107
brain
-
Rattus norvegicus
-
2.7.1.107
heart
low activity
Rattus norvegicus
-
2.7.1.107
kidney
low activity
Rattus norvegicus
-
2.7.1.107
liver
low activity
Rattus norvegicus
-
2.7.1.107
lung
-
Rattus norvegicus
-
2.7.1.107
spleen
-
Rattus norvegicus
-
2.7.1.107
thymus
-
Rattus norvegicus
-
2.7.1.107
uterus
low activity
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.7.1.107
5.187
-
enzyme form DGK IV
Rattus norvegicus
2.7.1.107
11.5
-
enzyne form DGK I
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.107
ATP + 1,2-diarachidonoyl-glycerol
-
640364
Rattus norvegicus
ADP + 1,2-diarachidonoyl-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol
-
640364
Rattus norvegicus
ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + sn-1,2-dioleoylglycerol
-
640364
Rattus norvegicus
ADP + sn-1,2-dioleoylglycerol 3-phosphate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.107
7.4
-
DGK I and DGK IV
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.107
6.5
8.5
pH 6.5: about 45% of maximal activity, pH 8.5: about 45% of maximal activity
Rattus norvegicus
2.7.1.107
6.8
9
pH 6.5: about 85% of maximal activity pH 9.0: about 50% of maximal activity, enzyme form DGK I
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.107
detergent
no activity in absence of detergent
Rattus norvegicus
2.7.1.107
phosphatidic acid
good activator of DGK IV, no effect on DGK I activity
Rattus norvegicus
2.7.1.107
phosphatidylcholine
moderate enhancement of DGK IV, no effect on DGK I activity
Rattus norvegicus
2.7.1.107
phosphatidylinositol
enhances activity of DGK I and DGK IV
Rattus norvegicus
2.7.1.107
phosphatidylserine
enhances activity of DGK I and DGK IV
Rattus norvegicus
2.7.1.107
sodium cholate
enhances activity of DGK I and DGK IV
Rattus norvegicus
2.7.1.107
sodium deoxycholate
enhances activity of DGK I and DGK IV
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.107
adenosine
0.5 mM, 5% inhibition of DGK I and 28% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
ADP
0.5 mM, 77% inhibition of DGK I and 66% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
AMP
0.5 mM, 18% inhibition of DGK I and 14% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
ATPgammaS
0.5 mM, 93% inhibition of DGK I and 71% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
cAMP
0.5 mM, 20% inhibition of DGK I and 15% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
CDP
0.5 mM, 43% inhibition of DGK I and 28% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
CTP
0.5 mM, 42% inhibition of DGK I and 9% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
GDP
0.5 mM, 74% inhibition of DGK I and 72% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
GTP
0.5 mM, 74% inhibition of DGK I and 56% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
GTPgammaS
0.5 mM, 37% inhibition of DGK I and 32% inhibition of DGK IV
Rattus norvegicus
2.7.1.107
quercetin
0.1 mM, 41% inhibition of DGK I and 15% inhibition of DGK IV
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.107
0.07
-
1-stearoyl-2-arachidonoyl-glycerol
pH 7.4, 30C, enzyme form DGK I
Rattus norvegicus
2.7.1.107
0.09
-
1,2-diarachidonoyl-glycerol
pH 7.4, 30C, enzyme form DGK I
Rattus norvegicus
2.7.1.107
0.09
-
1-stearoyl-2-arachidonoyl-glycerol
pH 7.4, 30C, enzyme form DGK IV
Rattus norvegicus
2.7.1.107
0.1
-
sn-1,2-dioleoylglycerol
pH 7.4, 30C, enzyme form DGK IV
Rattus norvegicus
2.7.1.107
0.125
-
ATP
pH 7.4, 30C, in presence of 1,2-dioleoylglycerol, enzyme form DGK I
Rattus norvegicus
2.7.1.107
0.125
-
sn-1,2-dioleoylglycerol
pH 7.4, 30C, enzyme form DGK I
Rattus norvegicus
2.7.1.107
0.14
-
1,2-diarachidonoyl-glycerol
pH 7.4, 30C, enzyme form DGK IV
Rattus norvegicus
2.7.1.107
0.25
-
ATP
pH 7.4, 30C, in presence of 1,2-dioleoylglycerol, enzyme form DGK IV
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.107
cytosol
DGK I
Rattus norvegicus
5829
-
2.7.1.107
membrane
bound to; DGK IV
Rattus norvegicus
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.107
Ca2+
DGK I: good activator, DGK IV: activation with 1 mM CaCl2 is comparable to that obtained on presence of MgCl2
Rattus norvegicus
2.7.1.107
Mg2+
DGK I: maximal activation at 20 mM. DGK IV: maximal activation at 5 mM
Rattus norvegicus
2.7.1.107
Mn2+
DGK I: activation is not so great as with MgCl2. Maximal stimulation at 0.1 mM. DGK IV: activation is 10fold less than with MgCl2
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.107
DGK I and DGK IV
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.107
brain
-
Rattus norvegicus
-
2.7.1.107
heart
low activity
Rattus norvegicus
-
2.7.1.107
kidney
low activity
Rattus norvegicus
-
2.7.1.107
liver
low activity
Rattus norvegicus
-
2.7.1.107
lung
-
Rattus norvegicus
-
2.7.1.107
spleen
-
Rattus norvegicus
-
2.7.1.107
thymus
-
Rattus norvegicus
-
2.7.1.107
uterus
low activity
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.7.1.107
5.187
-
enzyme form DGK IV
Rattus norvegicus
2.7.1.107
11.5
-
enzyne form DGK I
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.107
ATP + 1,2-diarachidonoyl-glycerol
-
640364
Rattus norvegicus
ADP + 1,2-diarachidonoyl-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol
-
640364
Rattus norvegicus
ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
-
-
?
2.7.1.107
ATP + sn-1,2-dioleoylglycerol
-
640364
Rattus norvegicus
ADP + sn-1,2-dioleoylglycerol 3-phosphate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.107
7.4
-
DGK I and DGK IV
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.107
6.5
8.5
pH 6.5: about 45% of maximal activity, pH 8.5: about 45% of maximal activity
Rattus norvegicus
2.7.1.107
6.8
9
pH 6.5: about 85% of maximal activity pH 9.0: about 50% of maximal activity, enzyme form DGK I
Rattus norvegicus