EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.107 | detergent | no activity in absence of detergent | Rattus norvegicus | |
2.7.1.107 | phosphatidic acid | good activator of DGK IV, no effect on DGK I activity | Rattus norvegicus | |
2.7.1.107 | phosphatidylcholine | moderate enhancement of DGK IV, no effect on DGK I activity | Rattus norvegicus | |
2.7.1.107 | phosphatidylinositol | enhances activity of DGK I and DGK IV | Rattus norvegicus | |
2.7.1.107 | phosphatidylserine | enhances activity of DGK I and DGK IV | Rattus norvegicus | |
2.7.1.107 | sodium cholate | enhances activity of DGK I and DGK IV | Rattus norvegicus | |
2.7.1.107 | sodium deoxycholate | enhances activity of DGK I and DGK IV | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.107 | adenosine | 0.5 mM, 5% inhibition of DGK I and 28% inhibition of DGK IV | Rattus norvegicus | |
2.7.1.107 | ADP | 0.5 mM, 77% inhibition of DGK I and 66% inhibition of DGK IV | Rattus norvegicus | |
2.7.1.107 | AMP | 0.5 mM, 18% inhibition of DGK I and 14% inhibition of DGK IV | Rattus norvegicus | |
2.7.1.107 | ATPgammaS | 0.5 mM, 93% inhibition of DGK I and 71% inhibition of DGK IV | Rattus norvegicus | |
2.7.1.107 | cAMP | 0.5 mM, 20% inhibition of DGK I and 15% inhibition of DGK IV | Rattus norvegicus | |
2.7.1.107 | CDP | 0.5 mM, 43% inhibition of DGK I and 28% inhibition of DGK IV | Rattus norvegicus | |
2.7.1.107 | CTP | 0.5 mM, 42% inhibition of DGK I and 9% inhibition of DGK IV | Rattus norvegicus | |
2.7.1.107 | GDP | 0.5 mM, 74% inhibition of DGK I and 72% inhibition of DGK IV | Rattus norvegicus | |
2.7.1.107 | GTP | 0.5 mM, 74% inhibition of DGK I and 56% inhibition of DGK IV | Rattus norvegicus | |
2.7.1.107 | GTPgammaS | 0.5 mM, 37% inhibition of DGK I and 32% inhibition of DGK IV | Rattus norvegicus | |
2.7.1.107 | quercetin | 0.1 mM, 41% inhibition of DGK I and 15% inhibition of DGK IV | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.107 | 0.07 | - |
1-stearoyl-2-arachidonoyl-sn-glycerol | pH 7.4, 30°C, enzyme form DGK I | Rattus norvegicus | |
2.7.1.107 | 0.09 | - |
1,2-diarachidonoyl-glycerol | pH 7.4, 30°C, enzyme form DGK I | Rattus norvegicus | |
2.7.1.107 | 0.09 | - |
1-stearoyl-2-arachidonoyl-sn-glycerol | pH 7.4, 30°C, enzyme form DGK IV | Rattus norvegicus | |
2.7.1.107 | 0.1 | - |
sn-1,2-dioleoylglycerol | pH 7.4, 30°C, enzyme form DGK IV | Rattus norvegicus | |
2.7.1.107 | 0.125 | - |
sn-1,2-dioleoylglycerol | pH 7.4, 30°C, enzyme form DGK I | Rattus norvegicus | |
2.7.1.107 | 0.125 | - |
ATP | pH 7.4, 30°C, in presence of 1,2-dioleoylglycerol, enzyme form DGK I | Rattus norvegicus | |
2.7.1.107 | 0.14 | - |
1,2-diarachidonoyl-glycerol | pH 7.4, 30°C, enzyme form DGK IV | Rattus norvegicus | |
2.7.1.107 | 0.25 | - |
ATP | pH 7.4, 30°C, in presence of 1,2-dioleoylglycerol, enzyme form DGK IV | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.107 | cytosol | DGK I | Rattus norvegicus | 5829 | - |
2.7.1.107 | membrane | bound to | Rattus norvegicus | 16020 | - |
2.7.1.107 | membrane | DGK IV | Rattus norvegicus | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.107 | Ca2+ | DGK I: good activator, DGK IV: activation with 1 mM CaCl2 is comparable to that obtained on presence of MgCl2 | Rattus norvegicus | |
2.7.1.107 | Mg2+ | DGK I: maximal activation at 20 mM. DGK IV: maximal activation at 5 mM | Rattus norvegicus | |
2.7.1.107 | Mn2+ | DGK I: activation is not so great as with MgCl2. Maximal stimulation at 0.1 mM. DGK IV: activation is 10fold less than with MgCl2 | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.107 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.107 | DGK I and DGK IV | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.107 | brain | - |
Rattus norvegicus | - |
2.7.1.107 | heart | low activity | Rattus norvegicus | - |
2.7.1.107 | kidney | low activity | Rattus norvegicus | - |
2.7.1.107 | liver | low activity | Rattus norvegicus | - |
2.7.1.107 | lung | - |
Rattus norvegicus | - |
2.7.1.107 | spleen | - |
Rattus norvegicus | - |
2.7.1.107 | thymus | - |
Rattus norvegicus | - |
2.7.1.107 | uterus | low activity | Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.107 | 5.187 | - |
enzyme form DGK IV | Rattus norvegicus |
2.7.1.107 | 11.5 | - |
enzyne form DGK I | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.107 | ATP + 1,2-diarachidonoyl-glycerol | - |
Rattus norvegicus | ADP + 1,2-diarachidonoyl-glycerol 3-phosphate | - |
? | |
2.7.1.107 | ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol | - |
Rattus norvegicus | ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
2.7.1.107 | ATP + sn-1,2-dioleoylglycerol | - |
Rattus norvegicus | ADP + sn-1,2-dioleoylglycerol 3-phosphate | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.107 | 7.4 | - |
DGK I and DGK IV | Rattus norvegicus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.107 | 6.5 | 8.5 | pH 6.5: about 45% of maximal activity, pH 8.5: about 45% of maximal activity | Rattus norvegicus |
2.7.1.107 | 6.8 | 9 | pH 6.5: about 85% of maximal activity pH 9.0: about 50% of maximal activity, enzyme form DGK I | Rattus norvegicus |