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Literature summary extracted from

  • Daleo, G.R.; Piras, M.M.; Piras, R.
    Diglyceride kinase activity of microtubules. Characterization and comparison with the protein kinase and ATPase activities associated with vinblastine-isolated tubulin of chick embryonic muscles (1976), Eur. J. Biochem., 68, 339-346.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.107 enzyme activity is destroyed by trypsin, no protection by substrate Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.107 deoxycholate 0.5%, 30% inhibition Gallus gallus
2.7.1.107 ethanol 0.5%, 20% inhibition Gallus gallus
2.7.1.107 GTP
-
 Gallus gallus
2.7.1.107 Nonidet 0.5%, 45% inhibition Gallus gallus
2.7.1.107 Triton N-101 0.5%, 45% inhibition Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.107 0.03
-
 GTP pH 6.6, 30°C Gallus gallus
2.7.1.107 0.16
-
 ATP pH 6.6, 30°C Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.107 microtubule
-
 Gallus gallus 5874
-
2.7.1.107 soluble
-
 Gallus gallus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.107 Ca2+ absolute requirement for a divalent ion. Mg2+ is more effective than Mn2+ or Ca2+ Gallus gallus
2.7.1.107 Mg2+ absolute requirement for a divalent ion. Mg2+ is more effective than Mn2+ or Ca2+. 50% stimulation by 0.4 mM Mg2+, maximal activity at 5-40 mM Gallus gallus
2.7.1.107 Mn2+ absolute requirement for a divalent ion. Mg2+ is more effective than Mn2+ or Ca2+. Mn2+ is more effective on the enzyme from microtubule than on the enzyme from supernatant Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.107 additional information
-
 the molecular weight of the peak fraction of enzyme from supernatant is 125000 Da and the average molecular weight is 160000 Da, suggesting that more than one species might be present Gallus gallus
2.7.1.107 75000
-
 microtubular enzyme, sucrose density gradient centrifugation Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Gallus gallus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.107 embryo muscle Gallus gallus
-
2.7.1.107 muscle embryonic Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 1,2-dipalmitoyl-sn-glycerol + GTP
-
 Gallus gallus GDP + 1,2-dipalmitoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1,2-dipalmitoyl-sn-glycerol
-
 Gallus gallus ADP + 1,2-dipalmitoyl-sn-glycerol 3-phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.107 43 45 preincubation of microtubules, 10 min, 50% loss of activity Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.107 6.1
-
 enzyme from supernantant fraction Gallus gallus
2.7.1.107 6.4
-
 enzyme from microtubule Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.107 4.5 7.5 pH 4.5: about 50% of maximal activity, pH 7.5: about 45% of maximal activity, enzyme from supernatant. pH 4.5: about 55% of maximal activity, pH 7.5: about 45% of maximal activity, enzyme from microtubuli Gallus gallus