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Literature summary extracted from

  • Garcia de Frutos, P.; Baanante, I.V.
    The muscle isoform of 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase of the teleost Sparus aurata: relationship with the liver isoform (1995), Arch. Biochem. Biophys., 321, 297-302.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.105 citrate
-
 Sparus aurata
2.7.1.105 additional information phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme, not skeletal muscle enzyme Sparus aurata
2.7.1.105 phosphoenolpyruvate
-
 Sparus aurata
2.7.1.105 sn-glycerol 3-phosphate most potent inhibitor of phosphorylated liver enzyme, phosphorylation enhances sensitivity; skeletal muscle enzyme is not sensitive to inhibition Sparus aurata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.105 0.21
-
 beta-D-fructose 2,6-bisphosphate pH 7.4, 30°C, in presence of phosphate Sparus aurata
2.7.1.105 0.223
-
 ATP pH 7.4, 30°C, presence of phosphate Sparus aurata
2.7.1.105 0.94
-
 beta-D-fructose 6-phosphate pH 7.4, 30°C, presence of phosphate Sparus aurata

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Sparus aurata
-
 teleost fish
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.105 phosphoprotein phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme, not skeletal muscle enzyme Sparus aurata

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105 skeletal muscle Sparus aurata

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.105 ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Sparus aurata
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.105 additional information
-
-
 Sparus aurata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Sparus aurata ADP + beta-D-fructose 2,6-bisphosphate
-
 r
2.7.1.105 additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Sparus aurata ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.105 30
-
 assay at Sparus aurata

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.105 0.12
-
 citrate pH 7.4, 30°C Sparus aurata
2.7.1.105 0.23
-
 phosphoenolpyruvate pH 7.4, 30°C Sparus aurata