Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Martín-Sanz, P.; Cascales, M.; Boscá, L.
    Characterization of 6-phosphofructo-2-kinase from foetal-rat liver (1992), Biochem. J., 281, 457-463.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.105 iodoacetamide
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.105 additional information phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme; phosphorylation of foetal liver enzyme by protein kinase C, but no effect on adult liver cells Rattus norvegicus
2.7.1.105 sn-glycerol 3-phosphate stimulates phosphatase activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.105 0.044
-
 beta-D-fructose 6-phosphate pH 8.5, 30°C, foetal liver enzyme Rattus norvegicus
2.7.1.105 0.047
-
 beta-D-fructose 6-phosphate pH 8.5, 30°C, adult liver enzyme Rattus norvegicus
2.7.1.105 0.19
-
 ATP pH 8.5, 30°C, foetal enzyme Rattus norvegicus
2.7.1.105 0.195
-
 ATP pH 8.5, 30°C, adult enzyme Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.105 Mg2+ MgATP2 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.105 55000
-
 2 * 55000, SDS-PAGE Rattus norvegicus
2.7.1.105 89100
-
 foetal liver, gel filtration Rattus norvegicus
2.7.1.105 100000
-
 adult liver, gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Rattus norvegicus
-
 foetal and adult
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.105 phosphoprotein phosphorylation of foetal liver enzyme by protein kinase C, but no effect on adult liver cells Rattus norvegicus
2.7.1.105 phosphoprotein phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.105 0.055
-
 adult Rattus norvegicus
2.7.1.105 0.08
-
 foetal Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.105 dimer 2 * 55000, SDS-PAGE Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.105 0.15
-
 sn-glycerol 3-phosphate pH 7.1 Rattus norvegicus