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Literature summary extracted from

  • Doerr, C.; Zaparty, M.; Tjaden, B.; Brinkmann, H.; Siebers, B.
    The hexokinase of the hyperthermophile Thermoproteus tenax. ATP-dependent hexokinases and ADP-dependent glucokinases, two alternatives for glucose phosphorylation in Archaea (2003), J. Biol. Chem., 278, 18744-18753.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.1 hxk gene encoding hexokinase is cloned, sequenced and expressed in Escherichia coli, member of the so-called ROK family, genomic organization of the orfX-hxk operon, hxk gene encodes a 301 amino acids protein Thermoproteus tenax

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 ADP
-
 Thermoproteus tenax
2.7.1.1 AMP
-
 Thermoproteus tenax
2.7.1.1 D-glucose 6-phosphate
-
 Thermoproteus tenax
2.7.1.1 diphosphate 1 mM, 70% inhibition Thermoproteus tenax
2.7.1.1 EDTA 2.5 mM, complete inhibition Thermoproteus tenax
2.7.1.1 phosphate
-
 Thermoproteus tenax
2.7.1.1 UDP
-
 Thermoproteus tenax

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.043
-
 D-mannose at 50°C, pH 7.5 Thermoproteus tenax
2.7.1.1 0.058
-
 D-glucose at 50°C, pH 7.5 Thermoproteus tenax
2.7.1.1 0.187
-
 2-deoxy-D-glucose at 50°C, pH 7.5 Thermoproteus tenax
2.7.1.1 0.29
-
 ATP at 50°C, pH 7.5 Thermoproteus tenax
2.7.1.1 0.734
-
 D-fructose at 50°C, pH 7.5 Thermoproteus tenax

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.1 Mg2+ strict requirement for divalent metal ions Thermoproteus tenax
2.7.1.1 Mn2+ strict requirement for divalent metal ions Thermoproteus tenax
2.7.1.1 Mn2+ Mg2+ can be partially replaced by Mn2+ Thermoproteus tenax

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 32543
-
 1 * 32543, calculated from the amino acid sequence, x * 33000, SDS-PAGE Thermoproteus tenax
2.7.1.1 33000
-
 1 * 32543, calculated from the amino acid sequence, x * 33000, SDS-PAGE Thermoproteus tenax
2.7.1.1 40000
-
 gel filtration Thermoproteus tenax

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.1 D-glucose + ATP Thermoproteus tenax carbohydrate metabolism D-glucose 6-phosphate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Thermoproteus tenax Q7ZAH1 Kra 1, i.e. DSM 2078
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 recombinant hexokinase, expressed in Escherichia coli Thermoproteus tenax

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 additional information
-
-
 Thermoproteus tenax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + 2-deoxy-D-glucose
-
 Thermoproteus tenax ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
2.7.1.1 D-fructose + ATP
-
 Thermoproteus tenax ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP preferred specificity for glucose Thermoproteus tenax ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP carbohydrate metabolism Thermoproteus tenax D-glucose 6-phosphate + ADP
-
 ?
2.7.1.1 D-mannose + ATP
-
 Thermoproteus tenax ADP + D-mannose 6-phosphate
-
 ?
2.7.1.1 GTP + D-glucose 18% of the activity with ATP Thermoproteus tenax GDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 ITP + D-glucose 40% of the activity with ATP Thermoproteus tenax IDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 additional information not: ADP Thermoproteus tenax ?
-
 ?
2.7.1.1 additional information not: ribose, xylose, phosphorylated hexoses, UDP, polyphosphates Thermoproteus tenax ?
-
 ?
2.7.1.1 additional information not: D-galactose Thermoproteus tenax ?
-
 ?
2.7.1.1 additional information not: CTP Thermoproteus tenax ?
-
 ?
2.7.1.1 UTP + D-glucose 17% of the activity with ATP Thermoproteus tenax UDP + D-glucose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.1 monomer 1 * 32543, calculated from the amino acid sequence, x * 33000, SDS-PAGE Thermoproteus tenax

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.1 50
-
 assay at Thermoproteus tenax

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 7.5
-
 assay at Thermoproteus tenax

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP
-
 Thermoproteus tenax