BRENDA - Enzyme Database

Eigenschaften der Hexokinase aus der SCP Hefe Candida maltosa H

Röber, B.; Stolle, J.; Reuter, G.; Z. Allg. Mikrobiol. 24, 619-627 (1984)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.1
galactose
activates isoenzyme Hex II
Candida maltosa
2.7.1.1
isocitrate
activates isoenzyme Hex II
Candida maltosa
2.7.1.1
lyxose
activates isoenzyme Hex II
Candida maltosa
2.7.1.1
mannose 6-phosphate
activates isoenzyme Hex II
Candida maltosa
2.7.1.1
phosphoenolpyruvate
activates isoenzyme Hex I
Candida maltosa
2.7.1.1
ribose
activates isoenzyme Hex II
Candida maltosa
2.7.1.1
xylose
activates isoenzyme Hex II
Candida maltosa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.1
ADP
-
Candida maltosa
2.7.1.1
D-mannose
-
Candida maltosa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.1
additional information
-
additional information
-
Candida maltosa
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.1
Mg2+
below 3 mM; required
Candida maltosa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.1
96000
-
Hex II, gel filtration
Candida maltosa
2.7.1.1
110000
-
gel filtration; Hex I
Candida maltosa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.1
D-glucose + ATP
Candida maltosa
isoenzymes are a target for effective control of glucose 6-phosphate formation by energy charge of the cell or by the intracellular level of ATP, ADP and glucose instead of allosteric interconversions of the enzyme
D-glucose 6-phosphate + ADP
-
Candida maltosa
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.1
Candida maltosa
-
H, isoenzymes Hex I and Hex II
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.1
isoenzymes Hex I and Hex II, partial
Candida maltosa
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.1
additional information
-
-
Candida maltosa
2.7.1.1
0.11
0.16
Hex I and II, at pH 8.2
Candida maltosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.1
D-glucose + ATP
specific for ATP
640232
Candida maltosa
ADP + D-glucose 6-phosphate
-
640232
Candida maltosa
?
2.7.1.1
D-glucose + ATP
isoenzymes are a target for effective control of glucose 6-phosphate formation by energy charge of the cell or by the intracellular level of ATP, ADP and glucose instead of allosteric interconversions of the enzyme
640232
Candida maltosa
D-glucose 6-phosphate + ADP
-
640232
Candida maltosa
?
2.7.1.1
D-mannose + ATP
-
640232
Candida maltosa
ADP + D-mannose 6-phosphate
-
-
-
?
2.7.1.1
additional information
not: UTP
640232
Candida maltosa
?
-
-
-
-
2.7.1.1
additional information
not: ITP
640232
Candida maltosa
?
-
-
-
-
2.7.1.1
additional information
not: GTP
640232
Candida maltosa
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.1
7.5
8.2
isoenzyme Hex I
Candida maltosa
2.7.1.1
7.5
8.5
isoenzyme Hex II
Candida maltosa
2.7.1.1
8.2
-
assay at
Candida maltosa
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.1
ATP
-
Candida maltosa
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.1
galactose
activates isoenzyme Hex II
Candida maltosa
2.7.1.1
isocitrate
activates isoenzyme Hex II
Candida maltosa
2.7.1.1
lyxose
activates isoenzyme Hex II
Candida maltosa
2.7.1.1
mannose 6-phosphate
activates isoenzyme Hex II
Candida maltosa
2.7.1.1
phosphoenolpyruvate
activates isoenzyme Hex I
Candida maltosa
2.7.1.1
ribose
activates isoenzyme Hex II
Candida maltosa
2.7.1.1
xylose
activates isoenzyme Hex II
Candida maltosa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.1
ATP
-
Candida maltosa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.1
ADP
-
Candida maltosa
2.7.1.1
D-mannose
-
Candida maltosa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.1
additional information
-
additional information
-
Candida maltosa
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.1
Mg2+
below 3 mM; required
Candida maltosa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.1
96000
-
Hex II, gel filtration
Candida maltosa
2.7.1.1
110000
-
gel filtration; Hex I
Candida maltosa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.1
D-glucose + ATP
Candida maltosa
isoenzymes are a target for effective control of glucose 6-phosphate formation by energy charge of the cell or by the intracellular level of ATP, ADP and glucose instead of allosteric interconversions of the enzyme
D-glucose 6-phosphate + ADP
-
Candida maltosa
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.1
isoenzymes Hex I and Hex II, partial
Candida maltosa
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.1
additional information
-
-
Candida maltosa
2.7.1.1
0.11
0.16
Hex I and II, at pH 8.2
Candida maltosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.1
D-glucose + ATP
specific for ATP
640232
Candida maltosa
ADP + D-glucose 6-phosphate
-
640232
Candida maltosa
?
2.7.1.1
D-glucose + ATP
isoenzymes are a target for effective control of glucose 6-phosphate formation by energy charge of the cell or by the intracellular level of ATP, ADP and glucose instead of allosteric interconversions of the enzyme
640232
Candida maltosa
D-glucose 6-phosphate + ADP
-
640232
Candida maltosa
?
2.7.1.1
D-mannose + ATP
-
640232
Candida maltosa
ADP + D-mannose 6-phosphate
-
-
-
?
2.7.1.1
additional information
not: UTP
640232
Candida maltosa
?
-
-
-
-
2.7.1.1
additional information
not: ITP
640232
Candida maltosa
?
-
-
-
-
2.7.1.1
additional information
not: GTP
640232
Candida maltosa
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.1
7.5
8.2
isoenzyme Hex I
Candida maltosa
2.7.1.1
7.5
8.5
isoenzyme Hex II
Candida maltosa
2.7.1.1
8.2
-
assay at
Candida maltosa