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Literature summary extracted from

  • Easterby, J.S.; Qadri, S.S.
    Hexokinase type II from rat skeletal muscle (1982), Methods Enzymol., 90, 11-15.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.1.1 hexokinase type II is extremely unstable, hexose or thiol stabilizes Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 ADP
-
 Rattus norvegicus
2.7.1.1 D-glucose 1,6-diphosphate
-
 Rattus norvegicus
2.7.1.1 D-glucose 6-phosphate
-
 Rattus norvegicus
2.7.1.1 phosphate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.05
-
 D-glucose at 30°C, pH 7.5 Rattus norvegicus
2.7.1.1 0.06
-
 D-mannose at 30°C, pH 7.5 Rattus norvegicus
2.7.1.1 0.06
-
 D-glucosamine at 30°C, pH 7.5 Rattus norvegicus
2.7.1.1 0.5
-
 ATP at 30°C, pH 7.5 Rattus norvegicus
2.7.1.1 0.9
-
 D-fructose at 30°C, pH 7.5 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.1 soluble hexokinase type II, largely soluble Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 100000
-
 sedimentation equilibrium Rattus norvegicus
2.7.1.1 106000
-
 gel filtration Rattus norvegicus
2.7.1.1 108000
-
 1 * 108000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Rattus norvegicus
-
 Wistar rats
-
2.7.1.1 Rattus norvegicus
-
 hexokinase type II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 hexokinase type II, 5330fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.1 skeletal muscle hexokinase type II, best source of enzyme Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 120
-
 hexokinase type II, at 30°C Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 D-fructose + ATP
-
 Rattus norvegicus ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 D-glucosamine + ATP
-
 Rattus norvegicus ADP + D-glucosamine 6-phosphate
-
 ?
2.7.1.1 D-glucose + ATP
-
 Rattus norvegicus ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 D-mannose + ATP
-
 Rattus norvegicus ADP + D-mannose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.1 monomer 1 * 108000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.1 30
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 7.5
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.1 0.015
-
 D-glucose 6-phosphate ATP Rattus norvegicus
2.7.1.1 0.026
-
 D-glucose 6-phosphate hexose Rattus norvegicus
2.7.1.1 3
-
 D-Glucose 1,6-bisphosphate
-
 Rattus norvegicus
2.7.1.1 3.5
-
 ADP
-
 Rattus norvegicus
2.7.1.1 5.3
-
 phosphate
-
 Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.1 Rattus norvegicus analytical isoelectric focusing
-
 5.7