Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ho, C.L.; Noji, M.; Saito, M.; Yamazaki, M.; Saito, K.
    Molecular characterization of plastidic phosphoserine aminotransferase in serine biosynthesis from Arabidopsis (1998), Plant J., 16, 443-452.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.52
-
 Spinacia oleracea
2.6.1.52 cDNA encoding PSAT, single gene mapped on the lower arm of chromosome 4, overexpressed in Escherichia coli AD494 and BL21 Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.52 L-cysteine
-
 Arabidopsis thaliana
2.6.1.52 L-cysteine
-
 Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.52 0.07
-
 L-glutamate pH 8.2, 25°C Arabidopsis thaliana
2.6.1.52 0.15
-
 L-glutamate pH 8.2, 25°C Spinacia oleracea
2.6.1.52 3
-
 3-phosphohydroxypyruvate pH 8.2, 25°C Spinacia oleracea
2.6.1.52 5
-
 3-phosphohydroxypyruvate pH 8.2, 25°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.52 plastid
-
 Spinacia oleracea 9536
-
2.6.1.52 plastid
-
 Arabidopsis thaliana 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.52 41000
-
 SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.52 L-glutamate + 3-phosphohydroxypyruvate Spinacia oleracea
-
 O-phospho-L-serine + 2-oxoglutarate
-
 r
2.6.1.52 L-glutamate + 3-phosphohydroxypyruvate Arabidopsis thaliana
-
 O-phospho-L-serine + 2-oxoglutarate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.52 Arabidopsis thaliana Q96255 ecotype Columbia
-
2.6.1.52 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.52 recombinant enzyme Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.52 root
-
 Arabidopsis thaliana
-
2.6.1.52 seedling
-
 Arabidopsis thaliana
-
2.6.1.52 shoot
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.52 L-glutamate + 3-phosphohydroxypyruvate
-
 Spinacia oleracea O-phospho-L-serine + 2-oxoglutarate
-
 r
2.6.1.52 L-glutamate + 3-phosphohydroxypyruvate
-
 Arabidopsis thaliana O-phospho-L-serine + 2-oxoglutarate
-
 r