EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.5 | expression of rat liver enzyme in Saccharomyces cerevisiae and Escherichia coli | Rattus norvegicus |
EC Number | General Stability | Organism |
---|---|---|
2.6.1.5 | pyridoxal 5'-phosphate protects against thermal inactivation | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.5 | Canaline | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.5 | 0.7 | - |
2-oxoglutarate | pH 7.2, 37°C | Rattus norvegicus | |
2.6.1.5 | 3.4 | - |
L-tyrosine | pH 7.2, 37°C | Rattus norvegicus | |
2.6.1.5 | 60 | - |
tryptophan | pH 7.2, 37°C | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.5 | 50000 | - |
2 * 50000, SDS-PAGE | Rattus norvegicus |
2.6.1.5 | 110000 | - |
recombinant expressed in Escherichia coli, ultracentrifugation | Rattus norvegicus |
2.6.1.5 | 150000 | - |
recombinant expressed in Escherichia coli, native gradient PAGE | Rattus norvegicus |
2.6.1.5 | 160000 | - |
recombinant expressed in Escherichia coli, gel filtration | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.5 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.5 | partial purification of enzyme expressed in Saccharomyces cerevisiae and Escherichia coli using affinity chromatography | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.6.1.5 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.5 | additional information | - |
- |
Rattus norvegicus |
2.6.1.5 | 416 | - |
enzyme expressed in Escherichia coli | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.5 | L-phenylalanine + 2-oxoglutarate | - |
Rattus norvegicus | phenylpyruvate + L-glutamate | - |
? | |
2.6.1.5 | L-tyrosine + 2-oxoglutarate | - |
Rattus norvegicus | 4-hydroxyphenylpyruvate + L-glutamate | - |
? | |
2.6.1.5 | tryptophan + 2-oxoglutarate | - |
Rattus norvegicus | 3-indole-2-oxopropanoate + L-glutamate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.5 | dimer | 2 * 50000, SDS-PAGE | Rattus norvegicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.5 | additional information | - |
pyridoxal 5'-phosphate protects against thermal inactivation | Rattus norvegicus |
2.6.1.5 | additional information | - |
the apoenzyme is thermally unstable: t1/2 of 2 min at 55°C | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.5 | 7.7 | - |
recombinant enzyme expressed in Escherichia coli | Rattus norvegicus |