BRENDA - Enzyme Database show

Purification and properties of L-alanine:4,5-dioxovalerate aminotransferase from Chlorella regularis

Shioi, Y.; Nagamine, M.; Sasa, T.; Arch. Biochem. Biophys. 234, 117-124 (1984)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.6.1.43
stability is improved by 10-15% glycerol
Chlorella regularis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
2-oxoglutarate
-
Chlorella regularis
2.6.1.43
4,5-Dioxopentanoate
substrate inhibition
Chlorella regularis
2.6.1.43
amino-oxyacetic acid
-
Chlorella regularis
2.6.1.43
glyoxylic acid
-
Chlorella regularis
2.6.1.43
imidazole
-
Chlorella regularis
2.6.1.43
pyruvate
-
Chlorella regularis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.12
-
4,5-Dioxopentanoate
pH 8.0, 37C
Chlorella regularis
2.6.1.43
1.3
-
glyoxylate
pH 8.0, 37C
Chlorella regularis
2.6.1.43
3
-
L-alanine
pH 8.0, 37C, glyoxylate as amino acceptor
Chlorella regularis
2.6.1.43
3.5
-
L-alanine
pH 8.0, 37C
Chlorella regularis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
68000
-
2 * 68000, SDS-PAGE
Chlorella regularis
2.6.1.43
126000
-
gel filtration
Chlorella regularis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Chlorella regularis
-
5-aminolevulinate + pyruvate
-
Chlorella regularis
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Chlorella regularis S-50
-
5-aminolevulinate + pyruvate
-
Chlorella regularis S-50
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.43
Chlorella regularis
-
S-50
-
2.6.1.43
Chlorella regularis S-50
-
S-50
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.43
-
Chlorella regularis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.6.1.43
2.68
-
-
Chlorella regularis
Storage Stability
EC Number
Storage Stability
Organism
2.6.1.43
4C, 10-15% glycerol, 20% loss of activity after 5 days
Chlorella regularis
2.6.1.43
4C, unstable
Chlorella regularis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
glycine + 4,5-dioxopentanoate
21% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + glyoxylate
-
-
-
ir
2.6.1.43
glycine + 4,5-dioxopentanoate
21% of the activity with L-alanine
640050
Chlorella regularis S-50
5-aminolevulinate + glyoxylate
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640050
Chlorella regularis
5-aminolevulinate + pyruvate
-
640050
Chlorella regularis
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640050
Chlorella regularis S-50
5-aminolevulinate + pyruvate
-
640050
Chlorella regularis S-50
ir
2.6.1.43
L-glutamate + 4,5-dioxopentanoate
37% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
2.6.1.43
L-glutamate + 4,5-dioxopentanoate
37% of the activity with L-alanine
640050
Chlorella regularis S-50
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
2.6.1.43
L-glutamine + 4,5-dioxopentanoate
16% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + 2-oxoglutaramate
-
-
-
-
2.6.1.43
L-lysine + 4,5-dioxopentanoate
15% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + 6-amino-2-oxohexanoate
-
-
-
ir
2.6.1.43
L-serine + 4,5-dioxopentanoate
33% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + 3-hydroxy-2-oxopropanoate
-
-
-
ir
2.6.1.43
L-serine + 4,5-dioxopentanoate
33% of the activity with L-alanine
640050
Chlorella regularis S-50
5-aminolevulinate + 3-hydroxy-2-oxopropanoate
-
-
-
ir
2.6.1.43
additional information
does not use 2-oxoglutarate as amino acceptor
640050
Chlorella regularis
?
-
-
-
-
2.6.1.43
additional information
does not use 2-oxoglutarate as amino acceptor
640050
Chlorella regularis S-50
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.43
dimer
2 * 68000, SDS-PAGE
Chlorella regularis
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.6.1.43
55
-
retains 75% activity after 1 h incubation
Chlorella regularis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.43
8
-
-
Chlorella regularis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.6.1.43
0.5
-
glyoxylate
pH 8.0, 37C
Chlorella regularis
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.6.1.43
Chlorella regularis
isoelectrofocusing column 110 ml and Pharmalyte gradient pH 3-10 at 4C for 45 h
-
4.8
General Stability (protein specific)
EC Number
General Stability
Organism
2.6.1.43
stability is improved by 10-15% glycerol
Chlorella regularis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
2-oxoglutarate
-
Chlorella regularis
2.6.1.43
4,5-Dioxopentanoate
substrate inhibition
Chlorella regularis
2.6.1.43
amino-oxyacetic acid
-
Chlorella regularis
2.6.1.43
glyoxylic acid
-
Chlorella regularis
2.6.1.43
imidazole
-
Chlorella regularis
2.6.1.43
pyruvate
-
Chlorella regularis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.6.1.43
0.5
-
glyoxylate
pH 8.0, 37C
Chlorella regularis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.12
-
4,5-Dioxopentanoate
pH 8.0, 37C
Chlorella regularis
2.6.1.43
1.3
-
glyoxylate
pH 8.0, 37C
Chlorella regularis
2.6.1.43
3
-
L-alanine
pH 8.0, 37C, glyoxylate as amino acceptor
Chlorella regularis
2.6.1.43
3.5
-
L-alanine
pH 8.0, 37C
Chlorella regularis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
68000
-
2 * 68000, SDS-PAGE
Chlorella regularis
2.6.1.43
126000
-
gel filtration
Chlorella regularis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Chlorella regularis
-
5-aminolevulinate + pyruvate
-
Chlorella regularis
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Chlorella regularis S-50
-
5-aminolevulinate + pyruvate
-
Chlorella regularis S-50
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.43
-
Chlorella regularis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.6.1.43
2.68
-
-
Chlorella regularis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.6.1.43
4C, 10-15% glycerol, 20% loss of activity after 5 days
Chlorella regularis
2.6.1.43
4C, unstable
Chlorella regularis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
glycine + 4,5-dioxopentanoate
21% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + glyoxylate
-
-
-
ir
2.6.1.43
glycine + 4,5-dioxopentanoate
21% of the activity with L-alanine
640050
Chlorella regularis S-50
5-aminolevulinate + glyoxylate
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640050
Chlorella regularis
5-aminolevulinate + pyruvate
-
640050
Chlorella regularis
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640050
Chlorella regularis S-50
5-aminolevulinate + pyruvate
-
640050
Chlorella regularis S-50
ir
2.6.1.43
L-glutamate + 4,5-dioxopentanoate
37% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
2.6.1.43
L-glutamate + 4,5-dioxopentanoate
37% of the activity with L-alanine
640050
Chlorella regularis S-50
5-aminolevulinate + 2-oxoglutarate
-
-
-
ir
2.6.1.43
L-glutamine + 4,5-dioxopentanoate
16% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + 2-oxoglutaramate
-
-
-
-
2.6.1.43
L-lysine + 4,5-dioxopentanoate
15% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + 6-amino-2-oxohexanoate
-
-
-
ir
2.6.1.43
L-serine + 4,5-dioxopentanoate
33% of the activity with L-alanine
640050
Chlorella regularis
5-aminolevulinate + 3-hydroxy-2-oxopropanoate
-
-
-
ir
2.6.1.43
L-serine + 4,5-dioxopentanoate
33% of the activity with L-alanine
640050
Chlorella regularis S-50
5-aminolevulinate + 3-hydroxy-2-oxopropanoate
-
-
-
ir
2.6.1.43
additional information
does not use 2-oxoglutarate as amino acceptor
640050
Chlorella regularis
?
-
-
-
-
2.6.1.43
additional information
does not use 2-oxoglutarate as amino acceptor
640050
Chlorella regularis S-50
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.43
dimer
2 * 68000, SDS-PAGE
Chlorella regularis
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.6.1.43
55
-
retains 75% activity after 1 h incubation
Chlorella regularis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.43
8
-
-
Chlorella regularis
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.6.1.43
Chlorella regularis
isoelectrofocusing column 110 ml and Pharmalyte gradient pH 3-10 at 4C for 45 h
-
4.8