BRENDA - Enzyme Database show

Biosynthesis of porphyrin precursors. Purification and characterization of mammalian L-alanine:gamma,delta-dioxovaleric acid aminotransferase

Varticovski, L.; Kushner, J.P.; Burnham, B.F.; J. Biol. Chem. 255, 3742-3747 (1980)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
2.6.1.43
-
Bos taurus
General Stability
EC Number
General Stability
Organism
2.6.1.43
freezing and thawing does not affect activity
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
2-oxoglutarate
-
Bos taurus
2.6.1.43
5-aminolevulinate
-
Bos taurus
2.6.1.43
methylglyoxal
-
Bos taurus
2.6.1.43
pyruvate
-
Bos taurus
2.6.1.43
succinate semialdehyde
-
Bos taurus
2.6.1.43
Tris-HCl
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.24
-
4,5-Dioxopentanoate
pH 6.9, 37°C
Bos taurus
2.6.1.43
3.7
-
L-alanine
pH 6.9, 37°C
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.43
cytosol
cytosol contains minimal enzymic activity
Bos taurus
5829
-
2.6.1.43
mitochondrion
-
Bos taurus
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
240000
-
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Bos taurus
-
5-aminolevulinate + pyruvate
-
-
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.43
Bos taurus
-
bovine
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.43
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.43
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.43
0.16
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
beta-alanine + 4,5-dioxopentanoate
23% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + malonic semialdehyde
-
-
-
ir
2.6.1.43
D-alanine + 4,5-dioxopentanoate
3% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + pyruvate
-
-
-
ir
2.6.1.43
delta-amino-n-valerate + 4,5-dioxopentanoate
10% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 5-oxo-n-pentanoate
-
-
-
ir
2.6.1.43
DL-alanine + 4,5-dioxopentanoate
89% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + pyruvate
-
-
-
ir
2.6.1.43
gamma-amino-n-butyrate + 4,5-dioxopentanoate
10% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + succinic semialdehyde
-
-
-
ir
2.6.1.43
glycine + 4,5-dioxopentanoate
9% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + glyoxylate
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640046
Bos taurus
5-aminolevulinate + pyruvate
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640046
Bos taurus
5-aminolevulinate + pyruvate
-
640046
Bos taurus
ir
2.6.1.43
L-lysine + 4,5-dioxopentanoate
9% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 6-amino-2-oxohexanoate
-
-
-
ir
2.6.1.43
L-ornithine + 4,5-dioxopentanoate
6% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 5-amino-2-oxopentanoate
-
-
-
ir
2.6.1.43
additional information
glutamate is no substrate, transamination of methylglyoxal yields aminoacetone, less than 1% efficient as amino acceptor as 4,5-dioxopentanoate
640046
Bos taurus
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.43
6.9
-
-
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.43
pyridoxal 5'-phosphate
-
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.43
pyridoxal 5'-phosphate
-
Bos taurus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.6.1.43
-
Bos taurus
General Stability (protein specific)
EC Number
General Stability
Organism
2.6.1.43
freezing and thawing does not affect activity
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.43
2-oxoglutarate
-
Bos taurus
2.6.1.43
5-aminolevulinate
-
Bos taurus
2.6.1.43
methylglyoxal
-
Bos taurus
2.6.1.43
pyruvate
-
Bos taurus
2.6.1.43
succinate semialdehyde
-
Bos taurus
2.6.1.43
Tris-HCl
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.43
0.24
-
4,5-Dioxopentanoate
pH 6.9, 37°C
Bos taurus
2.6.1.43
3.7
-
L-alanine
pH 6.9, 37°C
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.43
cytosol
cytosol contains minimal enzymic activity
Bos taurus
5829
-
2.6.1.43
mitochondrion
-
Bos taurus
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.43
240000
-
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.43
L-alanine + 4,5-dioxopentanoate
Bos taurus
-
5-aminolevulinate + pyruvate
-
-
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.43
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.43
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.43
0.16
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.43
beta-alanine + 4,5-dioxopentanoate
23% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + malonic semialdehyde
-
-
-
ir
2.6.1.43
D-alanine + 4,5-dioxopentanoate
3% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + pyruvate
-
-
-
ir
2.6.1.43
delta-amino-n-valerate + 4,5-dioxopentanoate
10% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 5-oxo-n-pentanoate
-
-
-
ir
2.6.1.43
DL-alanine + 4,5-dioxopentanoate
89% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + pyruvate
-
-
-
ir
2.6.1.43
gamma-amino-n-butyrate + 4,5-dioxopentanoate
10% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + succinic semialdehyde
-
-
-
ir
2.6.1.43
glycine + 4,5-dioxopentanoate
9% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + glyoxylate
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640046
Bos taurus
5-aminolevulinate + pyruvate
-
-
-
ir
2.6.1.43
L-alanine + 4,5-dioxopentanoate
-
640046
Bos taurus
5-aminolevulinate + pyruvate
-
640046
Bos taurus
ir
2.6.1.43
L-lysine + 4,5-dioxopentanoate
9% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 6-amino-2-oxohexanoate
-
-
-
ir
2.6.1.43
L-ornithine + 4,5-dioxopentanoate
6% of the activity with L-alanine
640046
Bos taurus
5-aminolevulinate + 5-amino-2-oxopentanoate
-
-
-
ir
2.6.1.43
additional information
glutamate is no substrate, transamination of methylglyoxal yields aminoacetone, less than 1% efficient as amino acceptor as 4,5-dioxopentanoate
640046
Bos taurus
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.43
6.9
-
-
Bos taurus