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Literature summary extracted from

  • Cooper, A.J.; Bruschi, S.A.; Conway, M.; Hutson, S.M.
    Human mitochondrial and cytosolic branched-chain aminotransferases are cysteine S-conjugate beta-lyases, but turnover leads to inactivation (2003), Biochem. Pharmacol., 65, 181-192.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.42 recombinant BCATm and BCATc Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.42 2-oxoisocaproate
-
 Homo sapiens
2.6.1.42 benzothiazolyl-L-cysteine inhibits the L-leucine-2-oxoglutarate tranamination reaction of both isoenzymes Homo sapiens
2.6.1.42 beta-chloro-L-alanine rapidly inactivated by the beta-lyase substrate Homo sapiens
2.6.1.42 S-(1,1,2,2-tetrafluoroethyl)-L-cysteine rapidly inactivated by the beta-lyase substrate Homo sapiens
2.6.1.42 S-(1,2-dichlorovinyl)-L-cysteine rapidly inactivated by the beta-lyase substrate Homo sapiens
2.6.1.42 S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine rapidly inactivated by the beta-lyase substrate Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.42 0.6
-
 beta-chloro-L-alanine pH 7.4, 23°C, recombinant BCATm Homo sapiens
2.6.1.42 8.4
-
 S-(1,1,2,2-tetrafluoroethyl)-L-cysteine pH 7.4, 23°C, recombinant BCATm Homo sapiens
4.4.1.13 0.6
-
 beta-chloro-L-alanine
-
 Homo sapiens
4.4.1.13 8.4
-
 S-(1,1,2,2-tetrafluoroethyl)-L-cysteine 23°C, pH 7.4 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.42 cytosol
-
 Homo sapiens 5829
-
2.6.1.42 mitochondrion
-
 Homo sapiens 5739
-
4.4.1.13 cytosol
-
 Homo sapiens 5829
-
4.4.1.13 mitochondrion
-
 Homo sapiens 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.42 41730
-
 2 * 41730, recombinant BCATm, homodimer Homo sapiens
2.6.1.42 43400
-
 2 * 43400, recombinant BCATc, homodimer Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Homo sapiens
-
 human
-
4.4.1.13 Homo sapiens
-
 enzymic activity of branched-chain aminotransferases
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.4.1.13 an L-cysteine-S-conjugate + H2O = a thiol + NH3 + pyruvate with an amino acid containing a good leaving group in the beta position, beta-elemination is greatly favoured over transamination Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 beta-chloro-L-alanine + ?
-
 Homo sapiens 3-chloropyruvate + ?
-
 ?
2.6.1.42 additional information recombinant human BCATm and BCATc have beta-lyase activity towards 3 toxic L-cysteine S-conjugates, S-(1,1,2,2-tetrafluoroethyl)-L-cysteine, S-(1,2-dichlorovinyl)-L-cysteine and S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine and toward 3-chloro-L-alanine, BCATm is also active toward benzothiazolyl-L-cysteine, pyruvate formed from beta-lyase substrates Homo sapiens ?
-
 ?
2.6.1.42 S-(1,1,2,2-tetrafluoroethyl)-L-cysteine + ?
-
 Homo sapiens S-(1,1,2,2-tetrafluoroethyl)-2-oxo-3-thiopropanoate + ?
-
 ?
2.6.1.42 S-(1,2-dichlorovinyl)-L-cysteine + ?
-
 Homo sapiens S-(1,2-dichlorovinyl)-2-oxo-3-thiopropanoate + ?
-
 ?
2.6.1.42 S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine + ?
-
 Homo sapiens S-(2-chloro-1,1,2-trifluoroethyl)-2-oxo-3-thiopropanoate + ?
-
 ?
4.4.1.13 beta-chloro-L-alanine
-
 Homo sapiens ?
-
 ?
4.4.1.13 L-leucine + alpha-ketoglutarate
-
 Homo sapiens ?
-
 ?
4.4.1.13 S-(1,1,2,2-tetrafluoroethyl)-L-cysteine
-
 Homo sapiens ?
-
 ?
4.4.1.13 S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine
-
 Homo sapiens ?
-
 ?
4.4.1.13 S-(E-1,2-Dichlorovinyl)-L-cysteine
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.42 dimer 2 * 43400, recombinant BCATc, homodimer Homo sapiens
2.6.1.42 dimer 2 * 41730, recombinant BCATm, homodimer Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 BCATc
-
 Homo sapiens
2.6.1.42 BCATm
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.42 pyridoxal 5'-phosphate
-
 Homo sapiens