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Literature summary extracted from

  • Roos, U.; Bormann, C.
    Purification and characterization of L-histidine aminotransferase from nikkomycin-producing Streptomyces tendae Tu901 (1993), J. Gen. Microbiol., 139, 2773-2778.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.38 10
-
pyruvate pH 8.0, 37°C Streptomyces tendae
2.6.1.38 25
-
L-histidine pH 8.0, 37°C Streptomyces tendae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.38 45000
-
2 * 45000, SDS-PAGE Streptomyces tendae
2.6.1.38 85000
-
gel filtration Streptomyces tendae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.38 imidazol-5-yl-pyruvate + L-glutamate Streptomyces tendae involved in nikkomycin biosynthesis, essential in the utilization of imidazol-L-lactate and imidazole pyruvate as carbon sources histidine + 2-oxoglutarate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.38 Streptomyces tendae
-
Tue901/AEC6, mutant hut-11
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.38
-
Streptomyces tendae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.38 0.866
-
-
Streptomyces tendae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.38 histidine + 2-oxocaproate
-
Streptomyces tendae imidazol-5-yl-pyruvate + L-norleucine
-
r
2.6.1.38 histidine + 2-oxoglutarate
-
Streptomyces tendae imidazol-5-yl-pyruvate + L-glutamate
-
r
2.6.1.38 histidine + 2-oxoisovalerate
-
Streptomyces tendae imidazol-5-yl-pyruvate + L-valine
-
r
2.6.1.38 histidine + 2-oxovalerate
-
Streptomyces tendae imidazol-5-yl-pyruvate + L-norvaline
-
r
2.6.1.38 imidazol-5-yl-pyruvate + L-glutamate involved in nikkomycin biosynthesis, essential in the utilization of imidazol-L-lactate and imidazole pyruvate as carbon sources Streptomyces tendae histidine + 2-oxoglutarate
-
r
2.6.1.38 L-histidine + 2-oxobutyrate
-
Streptomyces tendae imidazol-5-yl-pyruvate + 2-aminobutanoate
-
r
2.6.1.38 L-histidine + glyoxylate
-
Streptomyces tendae imidazol-5-yl-pyruvate + glycine
-
r
2.6.1.38 L-histidine + pyruvate ping pong reaction mechanism Streptomyces tendae imidazol-5-yl-pyruvate + L-alanine
-
r
2.6.1.38 additional information does not use aromatic amino acids as amino substrates, L-phenylalanine, L-tyrosine, L-tryptophan and D-histidine are no substrates, does not react with L-histidinol phosphate, no activity on gels towards L-alanine, L-leucine, L-lysine, L-glutamate and L-aspartate with pyruvate as amino acceptor, no activity towards L-aspartate with 2-oxoglutarate Streptomyces tendae ?
-
?

Subunits

EC Number Subunits Comment Organism
2.6.1.38 homodimer 2 * 45000, SDS-PAGE Streptomyces tendae

Synonyms

EC Number Synonyms Comment Organism
2.6.1.38 HISAT
-
Streptomyces tendae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.38 37
-
-
Streptomyces tendae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.38 7.2
-
-
Streptomyces tendae