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Literature summary extracted from

  • Badet, B.; Vermoote, P.; Haumont, P.Y.; Lederer, F.; Le Goffic, F.
    Glucosamine synthetase from Escherichia coli: purification, properties, and glutamine-utilizing site location (1987), Biochemistry, 26, 1940-1948.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.16 6-diazo-5-oxo-L-norleucine
-
 Escherichia coli
2.6.1.16 iodoacetamide
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.16 0.4
-
 L-glutamine pH 7.5, 37°C Escherichia coli
2.6.1.16 2
-
 D-fructose 6-phosphate pH 7.5, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.16 70800
-
 2 * 70800, SDS-PAGE Escherichia coli
2.6.1.16 170000 193000 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.16 Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.16 7.63
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Escherichia coli L-glutamate + D-glucosamine 6-phosphate
-
 ir

Subunits

EC Number Subunits Comment Organism
2.6.1.16 dimer 2 * 70800, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.16 7.2
-
 TES buffer Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.16 0.0028
-
 6-diazo-5-oxo-L-norleucine pH 7.5, room temperature Escherichia coli
2.6.1.16 12.6
-
 iodoacetamide pH 7.5, room temperature Escherichia coli