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Literature summary extracted from

  • Moriguchi, M.; Yamamoto, K.; Kawai, H.; Tochikura, T.
    The partial purification of by L-glutamine D-fructose 6-phosphate amidotransferase from Baker's yeast (1976), Agric. Biol. Chem., 40, 1655-1656.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.16 dithiothreitol 1 mM, activates 1.8fold Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
2.6.1.16 complete loss of activity after treatment with ammonium sulfate, 30-50% saturation Saccharomyces cerevisiae
2.6.1.16 dithiothreitol, D-fructose 6-phosphate, D-glucose 6-phosphate or L-glutamine, 1 mM, increases thermal stability at 37°C Saccharomyces cerevisiae
2.6.1.16 freezing and thawing has no effect on activity Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.16 Mercuric chloride 84% inhibition at 1 mM Saccharomyces cerevisiae
2.6.1.16 N-ethylmaleimide 78% inhibition at1 mM Saccharomyces cerevisiae
2.6.1.16 p-chloromercuribenzoate 84% inhibition at 0.1 mM Saccharomyces cerevisiae
2.6.1.16 UDP-N-acetylglucosamine 40% inhibition at 1 mM Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.16 L-glutamine + D-fructose 6-phosphate Saccharomyces cerevisiae
-
 L-glutamate + D-glucosamine 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.16 Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.16 0.00783
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Saccharomyces cerevisiae L-glutamate + D-glucosamine 6-phosphate
-
 ?
2.6.1.16 L-glutamine + D-fructose 6-phosphate specific for: L-glutamine Saccharomyces cerevisiae L-glutamate + D-glucosamine 6-phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.16 37
-
 90 min, complete loss of activity without stabilizing agent. Dithiothreitol, D-fructose 6-phosphate, D-glucose 6-phosphate or L-glutamine, 1 mM, increase thermal stability at 37°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.16 7
-
 potassium phosphate buffer Saccharomyces cerevisiae