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Literature summary extracted from

  • Walker, G.E.; Dunbar, B.; Hunter, I.S.; Nimmo, H.G.; Coggins, J.R.
    Evidence for a novel class of microbial 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase in Streptomyces rimosus and Neurospora crassa (1996), Microbiology, 142, 1973-1982.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.5.1.54 overnight dialysis against buffer containing 10 mM EDTA at 4°C results in 85% inactivation Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 EDTA
-
 Neurospora crassa
2.5.1.54 EDTA
-
 Streptomyces coelicolor
2.5.1.54 Trp Trp-sensitive isozyme Neurospora crassa
2.5.1.54 Trp Trp-sensitive isozyme Streptomyces coelicolor
2.5.1.54 Trp Trp-sensitive isozyme Streptomyces rimosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.092
-
 phosphoenolpyruvate Trp-sensitive isozyme, pH 7.0, 30°C Streptomyces coelicolor
2.5.1.54 0.195
-
 D-erythrose 4-phosphate Trp-sensitive isozyme, pH 7.0, 30°C Streptomyces coelicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 54000
-
 2 * 54000, SDS-PAGE Neurospora crassa
2.5.1.54 54000
-
 2 * 54000, SDS-PAGE Streptomyces coelicolor
2.5.1.54 54000
-
 2 * 54000, SDS-PAGE Streptomyces rimosus
2.5.1.54 107000
-
 Trp-sensitive isozyme, gel filtration Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Neurospora crassa
-
 tryptophan-sensitive isozyme
-
2.5.1.54 Streptomyces coelicolor
-
-
-
2.5.1.54 Streptomyces coelicolor
-
 Trp-sensitive isozyme
-
2.5.1.54 Streptomyces coelicolor A3(2)
-
-
-
2.5.1.54 Streptomyces rimosus
-
 Trp-sensitive isozyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 Trp-sensitive isozyme to homogeneity, 2000fold Neurospora crassa
2.5.1.54 Trp-sensitive isozyme to homogeneity, 2380fold, large scale Streptomyces coelicolor
2.5.1.54 Trp-sensitive isozyme to homogeneity, 3750fold Streptomyces rimosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 7.7
-
 purified Trp-sensitive isozyme Neurospora crassa
2.5.1.54 8.33
-
 purified Trp-sensitive isozyme Streptomyces coelicolor
2.5.1.54 11.02
-
 purified Trp-sensitive isozyme Streptomyces rimosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Neurospora crassa 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Streptomyces coelicolor 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Streptomyces rimosus 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Streptomyces coelicolor A3(2) 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.54 dimer 2 * 54000, SDS-PAGE Neurospora crassa
2.5.1.54 dimer 2 * 54000, SDS-PAGE Streptomyces coelicolor
2.5.1.54 dimer 2 * 54000, SDS-PAGE Streptomyces rimosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 30
-
 assay at Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 7
-
 assay at Streptomyces coelicolor
2.5.1.54 7
-
 assay at Streptomyces rimosus
2.5.1.54 7 7.4 Trp-sensitive isozyme Streptomyces coelicolor