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Literature summary extracted from

  • Ray, J.M.; Bauerle, R.
    Purification and properties of tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Escherichia coli (1991), J. Bacteriol., 173, 1894-1901.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.54 gene aroG, Phe-sensitive isozyme, overexpression in strain gene aroH, Trp-sensitive isozyme, overexpression in deficient strain AB3248 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 1,10-phenanthroline activity is restored by Fe2+ or Zn2+ Escherichia coli
2.5.1.54 EDTA activity is restored by Fe2+ or Zn2+ Escherichia coli
2.5.1.54 Phe Phe-sensitive isozyme Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Fe2+ stimulates Escherichia coli
2.5.1.54 Mn2+ stimulates Escherichia coli
2.5.1.54 additional information no effect by Ca2+, Co2+, Cu2+, Fe3+, Mg2+ Escherichia coli
2.5.1.54 Zn2+ stimulates Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 39000
-
 2 * 39000, recombinant Trp-sensitive isozyme, SDS-PAGE Escherichia coli
2.5.1.54 69000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Escherichia coli
-
 tryptophan-sensitive isozyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 5-6fold to homogeneity Escherichia coli
2.5.1.54 recombinant from overexpressing strain Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 13.1
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Escherichia coli 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.54 dimer 2 * 39000, recombinant Trp-sensitive isozyme, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 25
-
 assay at Escherichia coli