Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schnappauf, G.; Hartmann, M.; Künzler, M.; Braus, G.H.
    The two 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase isoenzymes from Saccharomyces cerevisiae show different kinetic modes of inhibition (1998), Arch. Microbiol., 169, 517-524.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.54 ARO4 gene, tyrosine-sensitive isozyme, overexpression from plasmid in strain RH1326 Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 EDTA Co2+, Zn2+, Cu2+, and Fe3+ restore activity Saccharomyces cerevisiae
2.5.1.54 Phe competitive with respect to D-erythrose 4-phosphate and non-competitive to phosphoenolpyruvate; Tyr-sensitive isozyme is less inhibited than the Phe-sensitive isozyme Saccharomyces cerevisiae
2.5.1.54 Tyr noncompetitive with respect to D-erythrose 4-phosphate, competitive with respect to phosphoenolpyruvate; Tyr-sensitive isozyme Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.125
-
 phosphoenolpyruvate Tyr-sensitive isozyme, pH 6.8 Saccharomyces cerevisiae
2.5.1.54 0.5
-
 D-erythrose 4-phosphate Tyr-sensitive isozyme, pH 6.8 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 additional information Tyr-sensitive isozyme, metal factor required Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 42000
-
 x * 42000, Tyr-sensitive isozyme, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Saccharomyces cerevisiae
-
 ARO3-encoded isozyme is a phenylalanine-sensitive isozyme
-
2.5.1.54 Saccharomyces cerevisiae
-
 ARO4-encoded isozyme is a tyrosine-sensitive isozyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 recombinantly overexpresed Tyr-sensitive isozyme, 7.5fold Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate sequential mechanism Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 8.24
-
 purified recombinant Tyr-sensitive isozyme Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.5.1.54 -20°C, 50% glycerol, stable for at least 4 weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Saccharomyces cerevisiae 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.54 ? x * 42000, Tyr-sensitive isozyme, SDS-PAGE Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.54 6.5 7
-
 Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.54 0.0009
-
 Tyr Tyr-sensitive isozyme, pH 6.8 Saccharomyces cerevisiae
2.5.1.54 0.27
-
 Phe Tyr-sensitive isozyme, pH 6.8 Saccharomyces cerevisiae