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Literature summary extracted from

  • Nimmo, G.A.; Coggins, J.R.
    Some kinetic properties of the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Neurospora crassa (1981), Biochem. J., 199, 657-665.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate competitive against D-erythrose 4-phosphate and phosphoenolpyruvate Neurospora crassa
2.5.1.54 EDTA phosphoenolpyruvate or 3-deoxy-D-arabino-heptulosonate 7-phosphate and Mn2+, Co2+, Ca2+ and Mg2+ protect Neurospora crassa
2.5.1.54 phosphate non-competitive with respect to both phosphoenolpyruvate and D-erythrose 4-phosphate Neurospora crassa
2.5.1.54 Trp non-competitive against phosphoenolpyruvate and competitive for D-erythrose 4-phosphate; Trp-sensitive isozyme Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 additional information metalloenzyme Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Neurospora crassa
-
 tryptophan-sensitive isozyme
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate rapid equilibrium ordered mechanism, in which phosphoenolpyruvate is the first substrate to bind and 3-deoxy-D-arabinoheptulosonate 7-phosphate is the second product to be released Neurospora crassa
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O in solution D-erythrose 4-phosphate forms dimers that are in slow equilibrium with monomers Neurospora crassa 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 25
-
 assay at Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 7.6
-
 assay at Neurospora crassa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.54 additional information
-
 additional information
-
 Neurospora crassa