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Literature summary extracted from

  • Friedrich, C.G.; Schlegel, H.G.
    Aromatic amino acid biosynthesis in Alcaligenes eutrophus H16. I. Properties and regulation of 3-deoxy-D-arabino heptulosonate 7-phosphate synthase (1975), Arch. Microbiol., 103, 133-140.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.54 loss of 50% activity by freezing and thawing the cell extract Cupriavidus necator
2.5.1.54 unstable during manipulations such as dialysis, dilution, ammonium sulfate fractionation, chromatography on DEAE-cellulose or Sephadex G-200 Cupriavidus necator

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 Co2+ weak Cupriavidus necator
2.5.1.54 Cu2+ weak Cupriavidus necator
2.5.1.54 EDTA no inhibition Cupriavidus necator
2.5.1.54 Hg2+ complete inhibition at 1 mM Cupriavidus necator
2.5.1.54 additional information no inhibition by Tyr and Phe Xanthobacter autotrophicus
2.5.1.54 Na2SO4 slightly Cupriavidus necator
2.5.1.54 Ni2+ weak Cupriavidus necator
2.5.1.54 Phe 0.5 mM, 60% inhibition; Phe-sensitive isozyme Cupriavidus necator
2.5.1.54 Phe no inhibition; Phe-sensitive isozyme; strain 12/60/X, no inhibition; strain H1, strain H20 and strain 3/2: cumulative inhibition of Tyr and Phe Xanthobacter autotrophicus
2.5.1.54 Tyr 0.5 mM, 20% inhibition; Tyr-sensitive isozyme Cupriavidus necator
2.5.1.54 Tyr strain 33/X strongly but not totally inhibited; strain H1, strain H20 and strain 3/2: cumulative inhibition of Tyr and Phe; Tyr-sensitive isozyme Xanthobacter autotrophicus
2.5.1.54 Zn2+
-
 Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.043
-
 phosphoenolpyruvate pH 7.8, 25°C Cupriavidus necator
2.5.1.54 0.055
-
 D-erythrose 4-phosphate pH 7.8, 25°C Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Cupriavidus necator no repression by the aromatic amino acids 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 no repression by the aromatic amino acids 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Alcaligenes faecalis
-
-
-
2.5.1.54 Cupriavidus necator
-
-
-
2.5.1.54 Cupriavidus necator
-
 basonym Alcaligenes eutrophus
-
2.5.1.54 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-
2.5.1.54 Xanthobacter autotrophicus
-
-
-
2.5.1.54 Xanthobacter autotrophicus 7C
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 partially Cupriavidus necator

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 0.026
-
 partially purified enzyme Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Alcaligenes faecalis 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Cupriavidus necator 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Xanthobacter autotrophicus 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O no repression by the aromatic amino acids Cupriavidus necator 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Xanthobacter autotrophicus 7C 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O no repression by the aromatic amino acids Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 25
-
 assay at Cupriavidus necator
2.5.1.54 40
-
-
 Cupriavidus necator

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.54 additional information
-
 phosphoenolpyruvate protects against inactivation Cupriavidus necator
2.5.1.54 50
-
 inactivation above Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 7.2 8.2
-
 Cupriavidus necator

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.54 6.5 9 pH 6.5: about 35% of maximal activity, pH 9.0: about 60% of maximal activity Cupriavidus necator