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Literature summary extracted from

  • Kawasaki, T.
    Thiamine phosphate pyrophosphorylase (1979), Methods Enzymol., 62, 69-73.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.3 acetyl phosphate 4 mM, 82.8% inhibition Escherichia coli
2.5.1.3 ADP 4 mM, 42.4% inhibition Escherichia coli
2.5.1.3 ATP 4 mM, 64.7% inhibition Escherichia coli
2.5.1.3 CTP 4 mM, 58.7% inhibition Escherichia coli
2.5.1.3 diphosphate 0.05 mM, 50% inhibition Escherichia coli
2.5.1.3 GTP 4 mM, 76.7% inhibition Escherichia coli
2.5.1.3 phosphocreatine 4 mM, 63.8% inhibition Escherichia coli
2.5.1.3 phosphoenolpyruvate 4 mM, 31.1% inhibition Escherichia coli
2.5.1.3 UTP 4 mM, 71.6% inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.3 0.0004
-
 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate
-
 Escherichia coli
2.5.1.3 0.00085
-
 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.3 Ca2+ stimulates, 20% as effective as Mg2+ Escherichia coli
2.5.1.3 Co2+ stimulates, 20% as effective as Mg2+ Escherichia coli
2.5.1.3 Mg2+ required Escherichia coli
2.5.1.3 Mg2+ Km: 0.063 mM Escherichia coli
2.5.1.3 Mg2+ most effective at 6 mM Escherichia coli
2.5.1.3 Mn2+ stimulates, at 6 mM equally effective as Mg2+ Escherichia coli
2.5.1.3 Zn2+ stimulates, 20% as effective as Mg2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.3 17000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.3 Escherichia coli
-
 K12, strain PT-R1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.3 0.065
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.5.1.3 -20°C, stable for several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.3 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
-
 Escherichia coli thiamine monophosphate + diphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.3 40
-
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.3 45
-
 5 min, without addition of both substrates, completely inactivated Escherichia coli