Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kawasaki, Y.
    Copurification of hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase of Saccharomyces cerevisiae: characterization of hydroxyethylthiazole kinase as a bifunctional enzyme in the thiamine biosynthetic pathway (1993), J. Bacteriol., 175, 5153-5158.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.3 PCMB 0.01 mM, 98.5% inhibition, inhibition prevented by addition of 2-mercaptoethanol in 10fold molar excess Saccharomyces cerevisiae
2.7.1.50 2-Aminohydroxyethylthiazole
-
 Saccharomyces cerevisiae
2.7.1.50 PCMB 0.01 mM, 98.5% inhibition, inhibition prevented by addition of 2-mercaptoethanol Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.50 0.0057
-
 5-(2-hydroxyethyl)-4-methylthiazole pH 7.5, 37°C Saccharomyces cerevisiae
2.7.1.50 0.79
-
 ATP pH 7.5, 37°C Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.3 Mg2+ required Saccharomyces cerevisiae
2.7.1.50 Co2+ 10 mM, stimulates as effectively as Mg2+ Saccharomyces cerevisiae
2.7.1.50 Mg2+ 10 mM, stimulates as effectively as Co2+ Saccharomyces cerevisiae
2.7.1.50 Mn2+ stimulates, 42.7% as effectively as Mg2+ Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.3 60000
-
 8 * 60000, SDS-PAGE, hydroxyethylthiazole kinase is a bifunctional enzyme with thiamine-phosphate pyrophosphorylase activity Saccharomyces cerevisiae
2.5.1.3 470000
-
 hydroxyethylthiazole kinase is a bifunctional enzyme with thiamine-phosphate pyrophosphorylase activity, gel filtration Saccharomyces cerevisiae
2.7.1.50 60000
-
 8 * 60000, bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity, SDS-PAGE Saccharomyces cerevisiae
2.7.1.50 470000
-
 bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity, gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.3 2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate Saccharomyces cerevisiae the bifunctional enzyme hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase catalyzes two sequential steps in the synthesis of thiamin monophosphate from hydroxyethylthiazole diphosphate + ?
-
 ?
2.7.1.50 ATP + 4-methyl-5-(2-hydroxyethyl)thiazole Saccharomyces cerevisiae the bifunctioinal enzyme hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase catalyzes two sequential steps in the synthesis of thiamin monophosphate from hydroxyethylthiazole ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.3 Saccharomyces cerevisiae
-
 mutant resistant to 2-amino-4-methyl-5-beta-hydroxyethylthiazole, an antimetabolite of 4-methyl-5-beta-hydroxyethylthiazole, deficient in activity of both EC 2.5.1.3 and EC 2.7.1.50
-
2.5.1.3 Saccharomyces cerevisiae
-
 IFO 10483
-
2.5.1.3 Saccharomyces cerevisiae
-
 bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity
-
2.7.1.50 Saccharomyces cerevisiae
-
 mutant resistant to 2-amino-4-methyl-5-beta-hydroxyethylthiazole, an antimetabolite of 4-methyl-5-beta-hydroxyethylthiazole, deficient in activity of both EC 2.5.1.3 and EC 2.7.1.50
-
2.7.1.50 Saccharomyces cerevisiae
-
 bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.3 bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity Saccharomyces cerevisiae
2.7.1.50 bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.3 0.269
-
-
 Saccharomyces cerevisiae
2.7.1.50 0.53
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.5.1.3 -80°C, about 64.4% loss of activity after 1 month Saccharomyces cerevisiae
2.7.1.50 -80°C, about 50% loss of activity after 1 month Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.3 2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate the bifunctional enzyme hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase catalyzes two sequential steps in the synthesis of thiamin monophosphate from hydroxyethylthiazole Saccharomyces cerevisiae diphosphate + ?
-
 ?
2.5.1.3 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
-
 Saccharomyces cerevisiae thiamine monophosphate + diphosphate
-
 ?
2.7.1.50 ADP + 4-methyl-5-(2-hydroxyethyl)thiazole 3.4% of the activity with ATP Saccharomyces cerevisiae AMP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
2.7.1.50 ATP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
 Saccharomyces cerevisiae ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
2.7.1.50 ATP + 4-methyl-5-(2-hydroxyethyl)thiazole the bifunctioinal enzyme hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase catalyzes two sequential steps in the synthesis of thiamin monophosphate from hydroxyethylthiazole Saccharomyces cerevisiae ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
2.7.1.50 ATP + 5-(2-hydroxyethyl)-4-methylthiazole
-
 Saccharomyces cerevisiae ADP + 4-methyl-5-(2-phosphoethyl)-thiazole
-
 ?
2.7.1.50 CTP + 4-methyl-5-(2-hydroxyethyl)thiazole 13.7% of the activity with ATP Saccharomyces cerevisiae CDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
2.7.1.50 dATP + 4-methyl-5-(2-hydroxyethyl)thiazole 24.8% of the activity with ATP Saccharomyces cerevisiae dADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
2.7.1.50 GTP + 4-methyl-5-(2-hydroxyethyl)thiazole 3.2% of the activity with ATP Saccharomyces cerevisiae GDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
2.7.1.50 UTP + 4-methyl-5-(2-hydroxyethyl)thiazole 75.1% of the activity with ATP Saccharomyces cerevisiae UDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.3 octamer 8 * 60000, SDS-PAGE, hydroxyethylthiazole kinase is a bifunctional enzyme with thiamine-phosphate pyrophosphorylase activity Saccharomyces cerevisiae
2.7.1.50 octamer 8 * 60000, bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.5.1.3 hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity Saccharomyces cerevisiae
2.7.1.50 hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.3 9.1
-
-
 Saccharomyces cerevisiae
2.7.1.50 7.7
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.3 7 9.1 pH 7.0: about 30% of maximal activity, pH 9.1: optimum Saccharomyces cerevisiae
2.7.1.50 7.4 9.2 pH 7.4: about 80% of activity maximum, pH 9.2: about 25% of activity maximum Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.50 0.0021
-
 2-Aminohydroxyethylthiazole pH 7.5, 37°C Saccharomyces cerevisiae