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Literature summary extracted from

  • Samejima, K.; Raina, A.; Yamanoha, B.; Eloranta, T.
    Purification of putrescine aminopropyltransferase (spermidine synthase) from eukaryotic tissues (1983), Methods Enzymol., 94, 270-276.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.16 5'-methylthioadenosine weak inhibition Bos taurus
2.5.1.16 5'-methylthioadenosine weak inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.16 0.01
-
 S-adenosyl-(5')-3-methylthio-1-propylamine pH 7.2, 37ºC Rattus norvegicus
2.5.1.16 0.01
-
 S-adenosyl-(5')-3-methylthio-1-propylamine pH 7.2, 37ºC Bos taurus
2.5.1.16 0.03
-
 1,4-diaminobutane pH 7.2, 37ºC Bos taurus
2.5.1.16 0.1
-
 1,4-diaminobutane pH 7.2, 37ºC Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.16 35800
-
 2 * 35800, SDS-PAGE Bos taurus
2.5.1.16 37000
-
 2 * 37000, SDS-PAGE Rattus norvegicus
2.5.1.16 73000
-
 gel filtration Rattus norvegicus
2.5.1.16 73000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Rattus norvegicus
-
 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Bos taurus
-
 5'-methylthioadenosine + spermidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Bos taurus
-
-
-
2.5.1.16 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16 extraction, ammonium sulfate fractionation, chromatography on DEAE-cellulose, affinity chromatography, gel filtration, 4480fold purified Rattus norvegicus
2.5.1.16 extraction, ammonium sulfate fractionation, chromatography on DEAE-Cellulose, chromatography on hydroxyapatite, chromatography on ATPA-Sepharose, gel filtration Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.16 brain
-
 Rattus norvegicus
-
2.5.1.16 brain
-
 Bos taurus
-
2.5.1.16 prostate gland ventral Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.16 0.712
-
-
 Bos taurus
2.5.1.16 1.3
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.5.1.16 0°C, in buffer containing 3 M NaCl, 1 mM DTT and 0.05-0.25 mM decarboxylated S-adenosylmethionine, several months Rattus norvegicus
2.5.1.16 0°C, in buffer containing 3 M NaCl, 1 mM DTT and 0.05-0.25 mM decarboxylated S-adenosylmethionine, several months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Rattus norvegicus 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Bos taurus 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,6-diaminohexane
-
 Rattus norvegicus ?
-
 ?
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,6-diaminohexane
-
 Bos taurus ?
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Rattus norvegicus 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Bos taurus 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane
-
 Rattus norvegicus 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane
-
 Bos taurus 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.16 dimer 2 * 37000, SDS-PAGE Rattus norvegicus
2.5.1.16 dimer 2 * 35800, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.16 37
-
 assay at Rattus norvegicus
2.5.1.16 37
-
 assay at Bos taurus