EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.15 | expression of recombinant enzyme in Escherichia coli | Pisum sativum |
2.7.6.3 | bifunctional protein 6-hydroxymethyl-7,8-dihydroxypterin pyrophosphokinase/7,8-dihydropteroate synthase is involved in tetrahydrofolate expressed in Escherichia coli | Pisum sativum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.15 | 7,8-Dihydropteroic acid | - |
Pisum sativum | |
2.5.1.15 | dihydrofolate monoglutamate | - |
Pisum sativum | |
2.5.1.15 | tetrahydrofolate monoglutamate | - |
Pisum sativum | |
2.7.6.3 | 6-hydroxymethyl-7,8-dihydropterin diphosphate | competitive inhibitor of ATP, mixed type inhibitor of 6-hydroxymethyl-7,8-dihydropteridine | Pisum sativum | |
2.7.6.3 | AMP | poor inhibitor | Pisum sativum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.15 | additional information | - |
additional information | activity in plant enzyme and recombinant enzyme | Pisum sativum | |
2.7.6.3 | 0.001 | - |
6-hydroxymethyl-7,8-dihydropteridine | bifunctional enzyme 6-hydroxymethyl-7,8-dihydroxypterin pyrophosphokinase/7,8-dihydropteroate synthase | Pisum sativum | |
2.7.6.3 | 0.002 | - |
6-hydroxymethyl-7,8-dihydropteridine | recombinant bifunctional enzyme 6-hydroxymethyl-7,8-dihydroxypterin pyrophosphokinase/7,8-dihydropteroate synthase expressed in Escherichia coli | Pisum sativum | |
2.7.6.3 | 0.07 | - |
ATP | bifunctional enzyme 6-hydroxymethyl-7,8-dihydroxypterin pyrophosphokinase/7,8-dihydropteroate synthase from Pisum sativum and recombinant enzyme expressed in Escherichia coli | Pisum sativum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.5.1.15 | mitochondrion | - |
Pisum sativum | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.15 | 53000 | - |
SDS-PAGE analysis of soluble protein | Pisum sativum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.15 | 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate | Pisum sativum | - |
diphosphate + 7,8-dihydropteroate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.15 | Pisum sativum | - |
- |
- |
2.7.6.3 | Pisum sativum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.15 | recombinant enzyme, affinity column | Pisum sativum |
2.7.6.3 | recombinant bifunctional protein 6-hydroxymethyl-7,8-dihydroxypterin pyrophosphokinase/7,8-dihydropteroate synthase is involved in tetrahydrofolate | Pisum sativum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.15 | leaf | - |
Pisum sativum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.15 | additional information | - |
specific activity in plant enzyme and recombinant enzyme | Pisum sativum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.15 | 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate | - |
Pisum sativum | diphosphate + 7,8-dihydropteroate | - |
? | |
2.7.6.3 | ATP + 6-hydroxymethyl-7,8-dihydropteridine | ordered bi bi mechanism where ATP binds first and 6-hydroxymethyl-7,8-dihydropterin diphosphate is released last | Pisum sativum | AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.6.3 | More | the bifunctional protein 6-hydroxymethyl-7,8-dihydroxypterin pyrophosphokinase/7,8-dihydropteroate synthase is involved in tetrahydrofolate synthesis | Pisum sativum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.6.3 | 6-hydroxymethyl-7,8-dihydroxypterin pyrophosphokinase/7,8-dihydropteroate synthase | bifunctional protein involved in tetrahydrofolate synthesis | Pisum sativum |
2.7.6.3 | HPPK/DHPS | - |
Pisum sativum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.15 | 30 | - |
assay at | Pisum sativum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.15 | additional information | - |
additional information | KI values of folate derivatives | Pisum sativum | |
2.7.6.3 | 0.005 | - |
6-hydroxymethyl-7,8-dihydropterin diphosphate | versus ATP | Pisum sativum | |
2.7.6.3 | 0.013 | - |
6-hydroxymethyl-7,8-dihydropterin diphosphate | versus 6-hydroxymethyl-7,8-dihydropteridine | Pisum sativum | |
2.7.6.3 | 0.4 | - |
AMP | versus 6-hydroxymethyl-7,8-dihydropteridine | Pisum sativum | |
2.7.6.3 | 0.7 | - |
AMP | versus ATP | Pisum sativum |