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Literature summary extracted from

  • Saunders, P.P.; Wilson, B.A.; Saunders, G.F.
    Purification and comparative properties of a pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus (1969), J. Biol. Chem., 244, 3691-3697.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.2 0.25
-
 uridine
-
 Geobacillus stearothermophilus
2.4.2.2 0.38
-
 thymidine
-
 Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.2 78000
-
 sedimentation velocity analysis Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.2 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.2
-
 Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.2 additional information
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.2 5-bromodeoxyuridine + phosphate 95% of the activity with uridine Geobacillus stearothermophilus 5-bromouracil + deoxyribose 1-phosphate
-
 ?
2.4.2.2 5-bromouridine + phosphate 95% of the activity with uridine Geobacillus stearothermophilus 5-bromouracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 cytidine + phosphate 8% of the activity with uridine Geobacillus stearothermophilus cytosine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 deoxyuridine + phosphate 179% of the activity with uridine, by measuring the conversion of uracil to uridine or deoxyuridine a slight preference for ribose 1-phosphate over deoxyribose 1-phosphate is observed Geobacillus stearothermophilus uracil + 2-deoxy-D-ribose 1-phosphate
-
 r
2.4.2.2 additional information no activity with deoxycytidine Geobacillus stearothermophilus ?
-
 ?
2.4.2.2 thymidine + phosphate 139% of the activity with uridine Geobacillus stearothermophilus thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate by measuring the conversion of uracil to uridine or deoxyuridine a slight preference for ribose 1-phosphate over deoxyribose 1-phosphate is observed Geobacillus stearothermophilus uracil + alpha-D-ribose 1-phosphate
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.2 60
-
 60 min, stable Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.2 7.2
-
 uridine, thymidine Geobacillus stearothermophilus