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Literature summary extracted from

  • Lienart, Y.; Comtat, J.; Barnoud, F.
    Wall-bound 1,3-beta-D-glucan:orthophosphate glucosyltransferase activity from Acacia cultured cells (1988), Plant Sci., 58, 165-170.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.97 0.25
-
 glucose
-
 Acacia verek
2.4.1.97 0.34
-
 laminaribiose phosphorolytic reaction Acacia verek
2.4.1.97 0.47
-
 Laminarin phosphorolytic reaction Acacia verek
2.4.1.97 1.45
-
 laminaribiose reaction with glucose 1-phosphate Acacia verek
2.4.1.97 1.7
-
 Laminarin reaction with glucose 1-phosphate Acacia verek

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.97 cell wall bound Acacia verek 5618
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.97 (1,3-beta-D-glucosyl)n + phosphate Acacia verek enzyme may be a key factor in the regulation of cell-wall extension and build up by switching hydrolase and synthase activity in a balance dependent manner (1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.97 Acacia verek
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.97 cell culture
-
 Acacia verek
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.97 (1,3-beta-D-glucosyl)n + phosphate in 50 mM citrate-phosphate buffer, pH 6.5, the phosphorolytic cleavage of laminarin and laminaribiose can be observed. In 0.02 M imidazole buffer, pH 6.5, the phosphorylase equilibrium lies in the direction of the synthesis reaction, alpha-D-glucose-1-phosphate as glucosyl donor is incorporated into laminaribiose Acacia verek (1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.97 (1,3-beta-D-glucosyl)n + phosphate enzyme may be a key factor in the regulation of cell-wall extension and build up by switching hydrolase and synthase activity in a balance dependent manner Acacia verek (1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.97 laminaribiose + glucose 1-phosphate in 50 mM citrate-phosphate buffer, pH 6.5, the phosphorolytic cleavage of laminarin and laminaribiose can be observed. In 0.02 M imidazole buffer, pH 6.5, the phosphorylase equilibrium lies in the direction of the synthesis reaction, alpha-D-glucose-1-phosphate as glucosyl donor is incorporated into laminaribiose Acacia verek phosphate + laminarin
-
 r