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Literature summary extracted from

  • Karlsson, O.P.; Dahlqvist, A.; Vikstroem, S.; Wieslander, A.
    Lipid dependence and basic kinetics of the purified 1,2-diacylglycerol 3-glucosyltransferase from membranes of Acholeplasma laidlawii (1997), J. Biol. Chem., 272, 929-936.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.337 CHAPS In CHAPS detergent, mixed micelles, enzyme shows a cooperative dependence on anionic lipids for activity Acholeplasma laidlawii
2.4.1.337 dioleoyl-phosphatidylglycerol stimulates the enzyme by an activating, potentially cooperative mechanism. Physiological concentrations of dioleoyl-phosphatidylglycerol influence the turnover number of the enzyme but not the interaction with UDP-glucose Acholeplasma laidlawii
2.4.1.337 sn-1,2-dioleoylglycerol activates Acholeplasma laidlawii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.337 0.37
-
 UDP-alpha-D-glucose pH 8.0, 28°C, presence of 31 mol% CHAPS Acholeplasma laidlawii
2.4.1.337 0.39
-
 UDP-alpha-D-glucose pH 8.0, 28°C, presence of 20 mol% CHAPS Acholeplasma laidlawii
2.4.1.337 0.44
-
 UDP-alpha-D-glucose pH 8.0, 28°C, presence of 25 mol% CHAPS Acholeplasma laidlawii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.337 membrane
-
 Acholeplasma laidlawii 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.337 Mg2+ strong dependence on, maximal activity at 15-28 mM MgCl2 Acholeplasma laidlawii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.337 40000
-
 1 * 40000, SDS-PAGE Acholeplasma laidlawii
2.4.1.337 40000
-
 x * 40000, SDS-PAGE Acholeplasma laidlawii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.337 UDPglucose + 1,2-diacylglycerol Acholeplasma laidlawii the product monoglucosyldiacylglycerol is the first glucolipid along the glucolipid pathway, and a major nonbilayer-prone lipid in the single membrane UDP + 3-D-glucosyl-1,2-diacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.337 Acholeplasma laidlawii
-
-
-
2.4.1.337 Acholeplasma laidlawii Q93P60
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.337
-
 Acholeplasma laidlawii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.337 12
-
-
 Acholeplasma laidlawii

Storage Stability

EC Number Storage Stability Organism
2.4.1.337 -20°C or -50°C, stable for at least 48 days Acholeplasma laidlawii
2.4.1.337 -20°C, stable for at least 48 days Acholeplasma laidlawii
2.4.1.337 20°C, 100 mM KH2PO4/K2HPO4, pH 8, 20% (v/v) glycerol, and 20 mM CHAPS, half-life 1.8 h Acholeplasma laidlawii
2.4.1.337 4°C, 100 mM KH2PO4/K2HPO4, pH 8, 20% (v/v) glycerol, and 20 mM CHAPS, half-life 50h Acholeplasma laidlawii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.337 UDP-alpha-D-glucose + 1,2-dioleoyl-sn-glycerol
-
 Acholeplasma laidlawii UDP + 1,2-dioleoyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
-
 ?
2.4.1.337 UDP-alpha-D-glucose + 1,2-dipalmitoyl-sn-glycerol
-
 Acholeplasma laidlawii UDP + 1,2-dipalmitoyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
-
 ?
2.4.1.337 UDP-glucose + 1,2-dioleoylglycerol
-
 Acholeplasma laidlawii UDP + 3-D-glucosyl-1,2-dioleoylglycerol
-
 ?
2.4.1.337 UDPglucose + 1,2-diacylglycerol
-
 Acholeplasma laidlawii UDP + 3-D-glucosyl-1,2-diacylglycerol
-
 ?
2.4.1.337 UDPglucose + 1,2-diacylglycerol the product monoglucosyldiacylglycerol is the first glucolipid along the glucolipid pathway, and a major nonbilayer-prone lipid in the single membrane Acholeplasma laidlawii UDP + 3-D-glucosyl-1,2-diacylglycerol
-
 ?
2.4.1.337 UDPglucose + 1,2-dipalmitoylglycerol twice as high enzyme activity as sn-1,2-dioleoylglycerol at all concentrations up to 2.5 mol% concentration Acholeplasma laidlawii UDP + 3-D-glucosyl-1,2-dipalmitoylglycerol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.337 ? 1 * 40000, SDS-PAGE Acholeplasma laidlawii
2.4.1.337 ? x * 40000, SDS-PAGE Acholeplasma laidlawii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.337 4
-
 half-life is around 50 h Acholeplasma laidlawii
2.4.1.337 20
-
 half-life is around 1.8 h Acholeplasma laidlawii