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Literature summary extracted from

  • Leonard, D.M.; Stachelek, S.J.; Safran, M.; Farwell, A.P.; Kowalik, T.F.; Leonard, J.L.
    Cloning, expression, and functional characterization of the substrate binding subunit of rat type II iodothyroine 5'-deiodinase (2000), J. Biol. Chem., 275, 25194-25201.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.21.99.4 dithiothreitol high concentrations of cofactor increases type I activity Rattus sp.
1.21.99.4 reduced thiols e.g. dithiothreitol Rattus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.99.4 expression in Escherichia coli via phage lambda infection Rattus sp.
1.21.99.4 overexpression in rat astrocytes of 29 kD subunit with and without GFP-tag via adenovirus infection Rattus sp.

General Stability

EC Number General Stability Organism
1.21.99.4 very short lifetime in vivo of isoenzyme type II (D2) from brain Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.99.4 additional information L-thyroxine inhibited competitively 3,3',5'-triiodothyronine utilization of isoenzyme type II Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.21.99.4 0.0067
-
 3,3',5'-triiodothyronine recombinant isoenzyme D2 (type II) from induced brain astrocytes with cosubstrate dithiothreitol KM: 18 mM Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.21.99.4 membrane bound, type II integral in neurolemmal membranes Rattus sp. 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.21.99.4 29000
-
 several subunits, thereof 1 * 29000 substrate binding subunit + 1 * 60000 cAMP-activation subunit + unknown number of other subunits, sequence analysis Rattus sp.
1.21.99.4 60000
-
 several subunits, thereof 1 * 29000 substrate binding subunit + 1 * 60000 cAMP-activation subunit + unknown number of other subunits, sequence analysis Rattus sp.
1.21.99.4 200000
-
-
 Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.99.4 3,3',5'-triiodothyronine + AH2 Rattus sp. isoenzyme type I 3,3'-diiodothyronine + iodide + A + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.99.4 Rattus sp.
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.21.99.4 3,3',5-triiodo-L-thyronine + iodide + acceptor + H+ = L-thyroxine + reduced acceptor type III, inner (tyrosyl) ring deiodination Rattus sp.
a
1.21.99.4 3,3',5-triiodo-L-thyronine + iodide + acceptor + H+ = L-thyroxine + reduced acceptor type II, outer ring (phenolic) deiodination Rattus sp.
a
1.21.99.4 3,3',5-triiodo-L-thyronine + iodide + acceptor + H+ = L-thyroxine + reduced acceptor type I, outer ring (phenolic) deiodination Rattus sp.
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.21.99.4 brain isoenzyme D2 Rattus sp.
-
1.21.99.4 glial cell i.e. brain astrocytes in culture Rattus sp.
-
1.21.99.4 glial cell isoenzyme type II (D2) Rattus sp.
-
1.21.99.4 kidney isoenzyme type I (D1) Rattus sp.
-
1.21.99.4 liver isoenzyme typ I (D1) Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.21.99.4 0.2
-
 isoenzyme D2 (type II) in cerebral cortex Rattus sp.
1.21.99.4 2
-
 isoenzyme D2 (type II) induced in cultured astrocytes Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.99.4 3,3',5'-triiodothyronine + AH2 isoenzyme type I Rattus sp. 3,3'-diiodothyronine + iodide + A + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.21.99.4 More several subunits, thereof 1 * 29000 substrate binding subunit + 1 * 60000 cAMP-activation subunit + unknown number of other subunits, sequence analysis Rattus sp.

Synonyms

EC Number Synonyms Comment Organism
1.21.99.4 iodothyronine 5'-deiodinase type II, no selenoprotein, induced by bt2cAMP and hydrocortisone (ED50 4nM) Rattus sp.
1.21.99.4 iodothyronine 5'-deiodinase type I, selenoprotein, inducible by T3 (higher synthesis) and angiotensin II (positive chronotropic effect in myocard) Rattus sp.
1.21.99.4 iodothyronine 5'-deiodinase isoenzymes type I (D1) and type II (D2) Rattus sp.