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Literature summary extracted from

  • Genet, R.; Donoyelle, C.; Menez, A.
    Purification and partial characterization of an amino acid alpha,beta-dehydrogenase, L-tryptophan 2',3'-oxidase from Chromobacterium violaceum (1994), J. Biol. Chem., 269, 18177-18184.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.10 5-hydroxy-L-tryptophan
-
 Chromobacterium violaceum
1.3.3.10 D-tryptophan
-
 Chromobacterium violaceum
1.3.3.10 methyl-3-indole
-
 Chromobacterium violaceum
1.3.3.10 additional information no inhibition with L-histidine, N-acetyl-L-phenylalaninamide and N-acetyl-L-tyrosinamide Chromobacterium violaceum
1.3.3.10 tryptamine
-
 Chromobacterium violaceum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.10 0.0195
-
 N-Acetyl-L-tryptophanamide
-
 Chromobacterium violaceum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.3.10 cytoplasm
-
 Chromobacterium violaceum 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.10 14000
-
 alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE Chromobacterium violaceum
1.3.3.10 74000
-
 alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE Chromobacterium violaceum
1.3.3.10 680000
-
 gel filtration Chromobacterium violaceum

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.10 Chromobacterium violaceum
-
 strain ATCC12472
-
1.3.3.10 Chromobacterium violaceum ATCC 12472
-
 strain ATCC12472
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.10 cell disruption with Eaton press, ultracentrifugation, ammonium sulfate precipitation, DEAE-column, concentration using centrifugal microcentrators, gel filtration, purified 108fold, yield 33.8% Chromobacterium violaceum

Storage Stability

EC Number Storage Stability Organism
1.3.3.10 -80°C, for several months and for 24-48 h at room temperature in the absence of any additives Chromobacterium violaceum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.10 N-acetyl-L-tryptophanamide + O2
-
 Chromobacterium violaceum N-acetyl-alpha,beta-didehydrotryptophanamide + H2O2
-
 ?
1.3.3.10 N-acetyl-L-tryptophanamide + O2
-
 Chromobacterium violaceum ATCC 12472 N-acetyl-alpha,beta-didehydrotryptophanamide + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.10 octamer alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE Chromobacterium violaceum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.3.10 80
-
-
 Chromobacterium violaceum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.3.10 0.753
-
 N-Acetyl-L-tryptophanamide
-
 Chromobacterium violaceum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.3.10 3 9.5
-
 Chromobacterium violaceum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.3.10 additional information NAD+, NADP+, FAD, or FMN show no effect on the activity Chromobacterium violaceum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.3.10 0.0013
-
 methyl-3-indole
-
 Chromobacterium violaceum
1.3.3.10 0.0054
-
 tryptamine
-
 Chromobacterium violaceum
1.3.3.10 0.0066
-
 D-tryptophan
-
 Chromobacterium violaceum
1.3.3.10 0.037
-
 5-hydroxy-L-tryptophan
-
 Chromobacterium violaceum