Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Thrower, J.S.; Blalock, R.; Klinman, J.P.
    Steady-state kinetics of substrate binding and iron release in tomato ACC oxidase (2001), Biochemistry, 40, 9717-9724.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.17.4 ascorbate required Solanum lycopersicum
1.14.17.4 CO2 activates Solanum lycopersicum
1.14.17.4 additional information no activation by bisulfite, isozyme AOC1 Solanum lycopersicum
1.14.17.4 O2 required Solanum lycopersicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.17.4 isozyme ACO1, overexpression in Escherichia coli BL21(DE3)-pLysS Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.17.4 1-aminocyclopropane-1-carboxylate slight inhibition at high concentration Solanum lycopersicum
1.14.17.4 Zn2+ competitive to Fe2+ Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.17.4 0.028
-
 O2 recombinant isozyme ACO1, in presence of 0.5 mM L-ascorbate and 20 mM CO2/HCO3-, pH 7.2, 25°C Solanum lycopersicum
1.14.17.4 0.077
-
 1-aminocyclopropane-1-carboxylate recombinant isozyme ACO1, in presence of 0.5 mM L-ascorbate and 20 mM CO2/HCO3-, pH 7.2, 25°C Solanum lycopersicum
1.14.17.4 4.6
-
 L-ascorbate recombinant isozyme ACO1, in presence of 0.5 mM L-ascorbate and 20 mM CO2/HCO3-, pH 7.2, 25°C Solanum lycopersicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.4 Fe2+ required Solanum lycopersicum
1.14.17.4 Fe2+ a single bound Fe2+ per monomer Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2 Solanum lycopersicum catalyses the final step in ethylene biosynthesis ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.4 Solanum lycopersicum
-
 isozyme ACO1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.17.4 recombinant isozyme ACO1 from Escherichia coli BL21(DE3)-pLysS, to near homogeneity Solanum lycopersicum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2 = ethene + cyanide + dehydroascorbate + CO2 + 2 H2O catalytic mechanism Solanum lycopersicum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2 catalyses the final step in ethylene biosynthesis Solanum lycopersicum ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 specific for the substrates Solanum lycopersicum ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 i.e. ACC Solanum lycopersicum ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.17.4 25
-
 assay at Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.17.4 7.2
-
 assay at Solanum lycopersicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.17.4 38.9
-
 1-aminocyclopropane-1-carboxylate recombinant isozyme ACO1, in presence of 5 mM L-ascorbate and 20 mM CO2/HCO3-, pH 7.2, 25°C Solanum lycopersicum