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Literature summary extracted from
- Expression and characterization of three tomato 1-aminocyclopropane-1-carboxylate oxidase cDNAs in yeast (1998), Eur. J. Biochem., 253, 20-26.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.17.4 | ascorbate | 3 isozymes: absolutely dependent on invitro, no requirement in vivo | Solanum lycopersicum |  |
| 1.14.17.4 | CO2 | absolutely dependent on | Solanum lycopersicum | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.17.4 | isozyme ACO1, DNA sequence determination and analysis, expression in Saccharomyces cerevisiae | Solanum lycopersicum |
| 1.14.17.4 | isozyme ACO2, DNA sequence determination and analysis, expression in Saccharomyces cerevisiae | Solanum lycopersicum |
| 1.14.17.4 | isozyme ACO3, DNA sequence determination and analysis, expression in Saccharomyces cerevisiae | Solanum lycopersicum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.17.4 | 1,10-phenanthroline | - |
Solanum lycopersicum | |
| 1.14.17.4 | Co2+ | - |
Solanum lycopersicum | |
| 1.14.17.4 | EDTA | - |
Solanum lycopersicum | |
| 1.14.17.4 | n-propyl gallate | free-radical scavenger; free-radical scavenger; free-radical scavenger | Solanum lycopersicum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.17.4 | 0.012 | - |
1-aminocyclopropane-1-carboxylate | recombinant isozyme ACO1, in presence of 3mM L-ascorbate, pH 7.2, 29°C | Solanum lycopersicum | |
| 1.14.17.4 | 0.013 | - |
1-aminocyclopropane-1-carboxylate | recombinant isozyme ACO1, in presence of 3mM L-ascorbate, pH 7.2, 29°C | Solanum lycopersicum | |
| 1.14.17.4 | 0.029 | - |
1-aminocyclopropane-1-carboxylate | recombinant isozyme ACO1, in presence of 3mM L-ascorbate and 10 mM HCO3-, pH 7.2, 29°C | Solanum lycopersicum | |
| 1.14.17.4 | 0.03 | - |
1-aminocyclopropane-1-carboxylate | recombinant isozyme ACO1, in presence of 3mM L-ascorbate, pH 7.2, 29°C | Solanum lycopersicum | |
| 1.14.17.4 | 0.03 | - |
1-aminocyclopropane-1-carboxylate | recombinant isozyme ACO1, in presence of 3mM L-ascorbate and 10 mM HCO3-, pH 7.2, 29°C | Solanum lycopersicum | |
| 1.14.17.4 | 0.067 | - |
1-aminocyclopropane-1-carboxylate | recombinant isozyme ACO1, in presence of 3mM L-ascorbate and 10 mM HCO3-, pH 7.2, 29°C | Solanum lycopersicum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.17.4 | Fe2+ | absolutely required | Solanum lycopersicum | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.17.4 | 1-aminocyclopropane-1-carboxylate + ascorbate + O2 | Solanum lycopersicum | - |
ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.17.4 | Solanum lycopersicum | P05116 | isozyme ACO1 | - |
| 1.14.17.4 | Solanum lycopersicum | P07920 | isozyme ACO2 | - |
| 1.14.17.4 | Solanum lycopersicum | P24157 | isozyme ACO3 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.17.4 | partial, recombinant from Saccharomyces cerevisiae | Solanum lycopersicum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.4 | 0.003 | - |
partially purified recombinant isozyme ACO2 | Solanum lycopersicum |
| 1.14.17.4 | 0.012 | - |
partially purified recombinant isozyme ACO3 | Solanum lycopersicum |
| 1.14.17.4 | 0.025 | - |
partially purified recombinant isozyme ACO1 | Solanum lycopersicum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.17.4 | 1-aminocyclopropane-1-carboxylate + ascorbate + O2 | - |
Solanum lycopersicum | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
| 1.14.17.4 | 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | i.e. ACC | Solanum lycopersicum | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.17.4 | ACO | - |
Solanum lycopersicum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.4 | 29 | - |
- |
Solanum lycopersicum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.4 | 6.8 | 7.2 | - |
Solanum lycopersicum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.4 | 5.8 | 8 | - |
Solanum lycopersicum |
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