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Literature summary extracted from

  • Hyodo, H.; Hashimoto, C.; Morozumi, S.; Hu, W.; Tanaka, K.
    Characterization and induction of the activity of 1-aminocyclopropane-1-carboxylate oxidase in the wounded mesocarp tissue of Cucurbita maxima (1993), Plant Cell Physiol., 34, 667-671.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.17.4 ascorbate required Cucurbita maxima
1.14.17.4 ascorbate optimal at 5-10 mM Cucurbita maxima
1.14.17.4 CO2 activates Cucurbita maxima
1.14.17.4 ethylene induction is inhibited by 2,5-norbornadiene Cucurbita maxima
1.14.17.4 ethylene stimulates, induction, regulation scheme Cucurbita maxima
1.14.17.4 additional information no activation by 2-oxoglutarate Cucurbita maxima
1.14.17.4 O2 required Cucurbita maxima

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.17.4 1,10-phenanthroline strong inhibition Cucurbita maxima
1.14.17.4 8-hydroxyquinoline
-
 Cucurbita maxima
1.14.17.4 Co2+
-
 Cucurbita maxima
1.14.17.4 Cu2+
-
 Cucurbita maxima
1.14.17.4 EDTA
-
 Cucurbita maxima
1.14.17.4 N2 complete inhibition of enzyme in anaerobic N2-atmosphere, reversible Cucurbita maxima
1.14.17.4 p-chloromercuribenzoate
-
 Cucurbita maxima
1.14.17.4 p-chloromercuriphenylsulfonate
-
 Cucurbita maxima
1.14.17.4 Zn2+
-
 Cucurbita maxima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.17.4 0.175
-
 1-aminocyclopropane-1-carboxylate under atmospheric conditions, pH 7.2, 30°C Cucurbita maxima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.4 Fe2+ required Cucurbita maxima
1.14.17.4 Fe2+ optimal at 0.025 mM Cucurbita maxima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2 Cucurbita maxima
-
 ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.4 Cucurbita maxima
-
 cvs. Ebisu and Miyako
-
1.14.17.4 Cucurbita maxima
-
 inducible by wounding and ethylene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.17.4 partial Cucurbita maxima

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.4 mesocarp wounded and incubated before enzyme extraction Cucurbita maxima
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2
-
 Cucurbita maxima ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 specific for the substrates Cucurbita maxima ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 i.e. ACC Cucurbita maxima ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.17.4 30
-
 assay at Cucurbita maxima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.17.4 7.2
-
 assay at Cucurbita maxima