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Literature summary extracted from

  • Dong, J.G.; Fernandez-Maculet, J.C.; Yang, S.F.
    Purification and characterization of 1-aminocyclopropane-1-carboxylate oxidase from apple fruit (1992), Proc. Natl. Acad. Sci. USA, 89, 9789-9793.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.17.4 ascorbate absolutely dependent on Malus domestica
1.14.17.4 ascorbate minimal 1 mM Malus domestica
1.14.17.4 CO2 absolutely dependent on Malus domestica
1.14.17.4 CO2 half-maximal activity at 0.5% atmospheric CO2 in vivo or 0.15 mM in the medium in vitro Malus domestica
1.14.17.4 dithiothreitol activates in presence of ascorbate, but cannot replace ascorbate Malus domestica
1.14.17.4 ethylene activation, in vivo and in vitro Malus domestica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.17.4 expressed in Escherichia coli Malus domestica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.17.4 alpha-aminoisobutyric acid
-
 Malus domestica
1.14.17.4 Co2+
-
 Malus domestica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.4 Fe2+ absolutely required Malus domestica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.17.4 35000
-
 1 * 35000, SDS-PAGE Malus domestica
1.14.17.4 39000
-
 gel filtration Malus domestica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2 Malus domestica absolutely dependent on CO2 ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.4 Malus domestica
-
 cv. Golden Delicious
-
1.14.17.4 Malus domestica
-
 inducible by ethylene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.17.4 180fold to near homogeneity Malus domestica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.4 fruit in climacteric stage Malus domestica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.17.4 0.02
-
 purified enzyme Malus domestica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2 absolutely dependent on CO2 Malus domestica ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 absolutely dependent on CO2 Malus domestica ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 i.e. ACC Malus domestica ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 absolutely dependent on ascorbate Malus domestica ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.17.4 monomer 1 * 35000, SDS-PAGE Malus domestica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.17.4 30
-
 assay at Malus domestica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.17.4 7.2
-
 assay at Malus domestica