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Literature summary extracted from

  • Heuberger, E.H.M.L.; Poolman, B.
    A spectroscopic assay for the analysis of carbohydrate transport reactions (2000), Eur. J. Biochem., 267, 228-234.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.5.2 analysis enzyme is used as coupling enzyme for monitoring carbohydrate-transport reactions, the method is particularly suited for determining transport reactions that are not coupled to any form of metabolic energy such as uniport reactions, or for characterizing mutant proteins with a defective energy-coupling mechanism or system with high-affinity constants for sugars Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.2 0.3
-
 D-glucose
-
 Acinetobacter calcoaceticus
1.1.5.2 0.66
-
 lactose
-
 Acinetobacter calcoaceticus
1.1.5.2 27
-
 melibiose
-
 Acinetobacter calcoaceticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.2 soluble
-
 Acinetobacter calcoaceticus
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Acinetobacter calcoaceticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 D-glucose + 2,6-dichlorophenol-indophenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 lactose + 2,6-dichlorophenol-indophenol
-
 Acinetobacter calcoaceticus ?
-
 ?
1.1.5.2 melibiose + 2,6-dichlorophenol-indophenol
-
 Acinetobacter calcoaceticus ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.2 pyrroloquinoline quinone prosthetic group Acinetobacter calcoaceticus