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Literature summary extracted from

  • Matsushita, K.; Shinagawa, E.; Adachi, O.; Ameyama, M.
    Quinoprotein D-glucose dehydrogenase of the Acinetobacter calcoaceticus respiratory chain: membrane-bound and soluble forms are different molecular species (1989), Biochemistry, 28, 6276-6280.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.2 0.074
-
 phenazine methosulfate membrane-bound enzyme form Acinetobacter calcoaceticus
1.1.5.2 1.9
-
 phenazine methosulfate soluble enzyme form Acinetobacter calcoaceticus
1.1.5.2 4.2
-
 D-glucose
-
 Acinetobacter calcoaceticus
1.1.5.2 24.5
-
 D-glucose soluble enzyme Acinetobacter calcoaceticus
1.1.5.2 26.7
-
 lactose soluble enzyme Acinetobacter calcoaceticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.2 membrane
-
 Acinetobacter calcoaceticus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.2 55000
-
 x * 55000, soluble enzyme SDS-PAGE Acinetobacter calcoaceticus
1.1.5.2 83000
-
 x * 83000, soluble enzyme, SDS-PAGE Acinetobacter calcoaceticus

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Acinetobacter calcoaceticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.2 soluble enzyme and membrane-bound enzyme Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.2 additional information
-
-
 Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 D-glucose + 2,6-dichlorophenol-indophenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + phenazine methosulfate
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.2 ? x * 55000, soluble enzyme SDS-PAGE Acinetobacter calcoaceticus
1.1.5.2 ? x * 83000, soluble enzyme, SDS-PAGE Acinetobacter calcoaceticus
1.1.5.2 dimer
-
 Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.2 6.5
-
 soluble enzyme Acinetobacter calcoaceticus
1.1.5.2 8.5
-
 membrane-bound enzyme Acinetobacter calcoaceticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.5.2 7.5 9.5 pH 7.5: about 40% of maximal activity, pH 9.5: about 85% of maximal activity, membrane-bound enzyme, reaction with phenazine methosulfate, 2,6-dichlorophenol indophenol and pyrroloquinoline quinone Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.2 pyrroloquinoline quinone prosthetic group Acinetobacter calcoaceticus