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Literature summary extracted from

  • Dumas, R.; Job, D.; Ortholand, J.Y.; Emeric, G.; Greiner, A.; Douce, R.
    Isolation and kinetic properties of acetohydroxy acid isomeroreductase from spinach (Spinacia oleracea) chloroplasts overexpressed in Escherichia coli (1992), Biochem. J., 288, 865-874.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.86 expression in Escherichia coli Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.86 2,3-dihydroxy-3-isovalerate
-
 Spinacia oleracea
1.1.1.86 2-hydroxy-2-methyl-3-oxopentanoate
-
 Spinacia oleracea
1.1.1.86 NADP+ product inhibition Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.86 0.0017
-
 NADPH pH 8.2, 30°C Spinacia oleracea
1.1.1.86 0.01
-
 2-acetolactate pH 8.2, 30°C Spinacia oleracea
1.1.1.86 0.01
-
 2-aceto-2-hydroxybutyrate pH 8.2, 30°C Spinacia oleracea
1.1.1.86 0.101
-
 NADH pH 8.2, 30°C Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.86 chloroplast
-
 Spinacia oleracea 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.86 Mg2+ required Spinacia oleracea
1.1.1.86 Mg2+ Km for Mg2+: 0.0045 mM, at pH 8.2, 30°C Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.86 56850
-
 2 * 56850, calculation from acid sequence Spinacia oleracea
1.1.1.86 57000
-
 2 * 57000, SDS-PAGE Spinacia oleracea
1.1.1.86 115000
-
 recombinant enzyme expressed in E. coli, gel filtration Spinacia oleracea
1.1.1.86 205000
-
 non-denaturing SDS-PAGE Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.86 2-acetolactate + NADPH Spinacia oleracea enzyme of branched chain amino acid synthesis 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.86 Spinacia oleracea
-
 overexpressed in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.86 recombinant enzyme from Escherichia coli Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.86 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.86 5
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.86 2-acetolactate + NADH + H+
-
 Spinacia oleracea 2,3-dihydroxy-3-methylbutanoate + NAD+ + H+
-
 r
1.1.1.86 2-acetolactate + NADPH enzyme of branched chain amino acid synthesis Spinacia oleracea 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 r
1.1.1.86 NADPH + 2-aceto-2-hydroxybutyrate
-
 Spinacia oleracea NADP+ + ?
-
 ?
1.1.1.86 NADPH + 2-acetolactate
-
 Spinacia oleracea NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.86 dimer 2 * 57000, SDS-PAGE Spinacia oleracea
1.1.1.86 dimer 2 * 56850, calculation from acid sequence Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.86 8.2
-
-
 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.86 NADPH
-
 Spinacia oleracea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.86 0.005
-
 NADP+ pH 8.2, 30°C, NADPH as variable substrate, 0.2 mM (2S)-2-acetolactate as fixed substrate Spinacia oleracea
1.1.1.86 0.0075
-
 NADP+ pH 8.2, 30°C, NADPH as variable substrate, 0.2 mM (2S)-2-aceto-2-hydroxybutanoate as fixed substrate Spinacia oleracea
1.1.1.86 0.095
-
 2,3-dihydroxyisovalerate pH 8.2, 30°C, (2S)-2-acetolactate as variable substrate, 0.250 mM NADPH as fixed substrate Spinacia oleracea
1.1.1.86 0.145
-
 2,3-dihydroxy-3-isovalerate pH 8.2, 30°C, (2S)-2-aceto-2-hydroxybutanoate as variable substrate, 0.250 mM NADPH as fixed substrate Spinacia oleracea
1.1.1.86 0.225
-
 NADP+ pH 8.2, 30°C, (2S)-2-aceto-2-hydroxybutanoate as variable substrate, 0.075 mM NADPH as fixed substrate Spinacia oleracea
1.1.1.86 0.24
-
 2-hydroxy-2-methyl-3-oxopentanoate pH 8.2, 30°C, (2S)-2-aceto-2-hydroxybutanoate as variable substrate, 0.075 mM NADPH as fixed substrate Spinacia oleracea
1.1.1.86 0.245
-
 2-hydroxy-2-methyl-3-oxopentanoate pH 8.2, 30°C, (2S)-2-acetolactate as variable substrate, 0.075 mM NADPH as fixed substrate Spinacia oleracea
1.1.1.86 0.33
-
 NADP+ pH 8.2, 30°C, (2S)-2-acetolactate as variable substrate, 0.075 mM NADPH as fixed substrate Spinacia oleracea
1.1.1.86 0.935
-
 2,3-dihydroxyisovalerate pH 8.2, 30°C, NADPH as variable substrate, 0.2 mM (2S)-2-acetolactate as fixed substrate Spinacia oleracea
1.1.1.86 1.5
-
 2,3-dihydroxyisovalerate pH 8.2, 30°C, NADPH as variable substrate, 0.2 mM (2S)-2-aceto-2-hydroxybutanoate as fixed substrate Spinacia oleracea
1.1.1.86 1.7
-
 2-hydroxy-2-methyl-3-oxopentanoate pH 8.2, 30°C, NADPH as variable substrate, 0.2 mM (2S)-2-acetolactate as fixed substrate Spinacia oleracea
1.1.1.86 3.6
-
 2-hydroxy-2-methyl-3-oxopentanoate pH 8.2, 30°C, NADPH as variable substrate, 0.2 mM (2S)-2-aceto-2-hydroxybutanoate as fixed substrate Spinacia oleracea