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Literature summary extracted from

  • Hofler, J.G.; Decedue, C.J.; Luginbuhl, G.H.; Reynolds, J.A.; Burns, R.O.
    The subunit structure of alpha-acetohydroxyacid isomeroreductase from Salmonella typhimurium (1975), J. Biol. Chem., 250, 877-882.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.86 0.015
-
 NADPH 30°C with 2-acetolactate or 2-aceto-2-hydroxybutyrate as substrate Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.86 0.29
-
 2-acetolactate 30°C, with 2-acetolactate or 2-aceto-2-hydroxybutyrate as substrate Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.86 0.78
-
 2-aceto-2-hydroxybutyrate 30°C Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.86 Mg2+ required Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.86 Mg2+ Km: 0.77 mM Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.86 57000
-
 4 * 57000, equilibrium sedimentation after treatment with 0.1 M 2-mercaptoethanol and 6 M guanidine hydrochloride Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.86 220000
-
 sedimentation equilibrium centrifugation Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.86 2-acetolactate + NADPH Salmonella enterica subsp. enterica serovar Typhimurium enzyme of branched chain amino acid synthesis 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.86 Salmonella enterica subsp. enterica serovar Typhimurium
-
 strain ATCC 15277
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.86
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.86 4.94
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.86 2-acetolactate + NADPH enzyme of branched chain amino acid synthesis Salmonella enterica subsp. enterica serovar Typhimurium 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 r
1.1.1.86 NADPH + 2-aceto-2-hydroxybutyrate
-
 Salmonella enterica subsp. enterica serovar Typhimurium NADP+ + ?
-
 ?
1.1.1.86 NADPH + 2-acetolactate
-
 Salmonella enterica subsp. enterica serovar Typhimurium NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.86 tetramer 4 * 57000, equilibrium sedimentation after treatment with 0.1 M 2-mercaptoethanol and 6 M guanidine hydrochloride Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.86 18.3
-
 2-acetolactate 30°C Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.86 78.3
-
 2-aceto-2-hydroxybutyrate 30°C Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.86 NADPH
-
 Salmonella enterica subsp. enterica serovar Typhimurium