EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.85 | study of the mutant enzymes G240A and L246E/V249M by X-ray crystallography | Thermus thermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.85 | G240A | decreased thermostability | Thermus thermophilus |
1.1.1.85 | L246E/V249M | decreased thermostability | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.85 | Thermus thermophilus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.85 | (2R,3S)-3-isopropylmalate + NAD+ | - |
Thermus thermophilus | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.85 | 87 | - |
the extended hydrogen bonds within the beta-sheet are the major reason for the decreased thermostability of G240A | Thermus thermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.85 | NAD+ | - |
Thermus thermophilus |