EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.85 | E87G | mutation does not affect the dissociation constant from the binary complex, but does greatly increase the KM-values | Thermus thermophilus |
1.1.1.85 | E87Q | mutation does not affect the dissociation constant from the binary complex, but does greatly increase the KM-values | Thermus thermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.85 | isocitrate | - |
Thermus thermophilus | |
1.1.1.85 | NADH | NADH always produces competitive inhibition patterns with respect to NAD and noncompetitive inhibition patterns with respect to the substrates | Thermus thermophilus | |
1.1.1.85 | Tartrate | - |
Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.85 | 0.000017 | - |
isopropylmalate | pH 7.3, 21°C, cofactor: NAD+, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 0.00048 | - |
isopropylmalate | pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87G | Thermus thermophilus | |
1.1.1.85 | 0.00298 | - |
isopropylmalate | pH 7.3, 21°C, cofactor: NADP+, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 0.00321 | - |
NAD+ | pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 0.0053 | - |
isopropylmalate | pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87Q | Thermus thermophilus | |
1.1.1.85 | 0.0326 | - |
malate | pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87Q | Thermus thermophilus | |
1.1.1.85 | 0.0407 | - |
Tartrate | pH 7.3, 21°C, cofactor: NAD+, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 0.0454 | - |
NAD+ | pH 7.3, 21°C, reaction with tartrate, wild-type enzym | Thermus thermophilus | |
1.1.1.85 | 0.1065 | - |
NAD+ | pH 7.3, 21°C, reaction with malate, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 0.131 | - |
NAD+ | pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87G | Thermus thermophilus | |
1.1.1.85 | 0.181 | - |
NAD+ | pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87Q | Thermus thermophilus | |
1.1.1.85 | 0.386 | - |
NAD+ | pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87G | Thermus thermophilus | |
1.1.1.85 | 0.761 | - |
Tartrate | pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87Q | Thermus thermophilus | |
1.1.1.85 | 0.778 | - |
NAD+ | pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87Q | Thermus thermophilus | |
1.1.1.85 | 0.817 | - |
Tartrate | pH 7.3, 21°C, cofactor: NADP+, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 1.21 | - |
Tartrate | pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87G | Thermus thermophilus | |
1.1.1.85 | 1.51 | - |
malate | pH 7.3, 21°C, cofactor: NAD+, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 1.573 | - |
NADP+ | pH 7.3, 21°C, reaction with tartrate, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 1.836 | - |
NADP+ | pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 1.853 | - |
NAD+ | pH 7.3, 21°C, reaction with malate, mutant enzyme E87Q | Thermus thermophilus | |
1.1.1.85 | 2.584 | - |
NAD+ | pH 7.3, 21°C, reaction with malate, mutant enzyme E87G | Thermus thermophilus | |
1.1.1.85 | 6.579 | - |
NADP+ | pH 7.3, 21°C, recation with malate, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 31.56 | - |
malate | pH 7.3, 21°C, cofactor: NADP+, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 35.59 | - |
malate | pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87G | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.85 | Thermus thermophilus | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.85 | (2R,3S)-3-isopropylmalate + NAD+ = (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | the oxidative decarboxylation of 2-hydroxy acid substrates by the enzyme occurs via a random kinetic mechanism with the formation of two abortive complexes: enzyme-NADH-substrate and enzyme-NADH-inhibitor | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.85 | (2R,3S)-3-isopropylmalate + NAD+ | - |
Thermus thermophilus | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
1.1.1.85 | malate + NAD+ | - |
Thermus thermophilus | pyruvate + NADH + H+ + CO2 | - |
? | |
1.1.1.85 | malate + NADP+ | - |
Thermus thermophilus | pyruvate + NADPH + CO2 | - |
? | |
1.1.1.85 | tartrate + NAD+ | - |
Thermus thermophilus | ? | - |
? | |
1.1.1.85 | tartrate + NADP+ | - |
Thermus thermophilus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.85 | dimer | 2 * 35000-40000, SDS-PAGE | Thermus thermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.85 | 0.1 | - |
Tartrate | pH 7.3, 21°C, reaction with NADP+, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 0.1 | - |
NADP+ | pH 7.3, 21°C, reaction with tartrate, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 0.19 | - |
Tartrate | pH 7.3, 21°C, reaction with NAD+, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 0.19 | - |
malate | pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q | Thermus thermophilus | |
1.1.1.85 | 0.19 | - |
NAD+ | pH 7.3, 21°C, reaction with tartrate, wild-type enzyme or reaction with malate, mutant enzyme E87Q | Thermus thermophilus | |
1.1.1.85 | 0.2 | - |
isopropylmalate | pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G | Thermus thermophilus | |
1.1.1.85 | 0.2 | - |
NAD+ | pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme E87G | Thermus thermophilus | |
1.1.1.85 | 0.21 | - |
isopropylmalate | pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q | Thermus thermophilus | |
1.1.1.85 | 0.21 | - |
Tartrate | pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G | Thermus thermophilus | |
1.1.1.85 | 0.21 | - |
NAD+ | pH 7.3, 21°C, reaction with tartrate, mutant enzyme E87G | Thermus thermophilus | |
1.1.1.85 | 0.21 | - |
NAD+ | pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme E87Q | Thermus thermophilus | |
1.1.1.85 | 0.24 | - |
isopropylmalate | pH 7.3, 21°C, reaction with NAD+, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 0.24 | - |
NAD+ | pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 0.26 | - |
Tartrate | pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q | Thermus thermophilus | |
1.1.1.85 | 0.26 | - |
malate | pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G | Thermus thermophilus | |
1.1.1.85 | 0.26 | - |
NAD+ | pH 7.3, 21°C, reaction with malate, mutant enzyme E87G or reaction with tartrate, mutant enzyme E87Q | Thermus thermophilus | |
1.1.1.85 | 1.66 | - |
isopropylmalate | pH 7.3, 21°C, reaction with NADP+, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 1.66 | - |
NADP+ | pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 4.69 | - |
malate | pH 7.3, 21°C, reaction with NADP+, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 4.69 | - |
NADP+ | pH 7.3, 21°C, reaction with malate, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 10.6 | - |
malate | pH 7.3, 21°C, reaction with NAD+, wild-type enzyme | Thermus thermophilus | |
1.1.1.85 | 10.6 | - |
NAD+ | pH 7.3, 21°C, reaction with malate, wild-type enzyme | Thermus thermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.85 | NAD+ | - |
Thermus thermophilus | |
1.1.1.85 | NADP+ | - |
Thermus thermophilus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.85 | additional information | - |
additional information | - |
Thermus thermophilus |