EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.85 | construction of a chimeric gene by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, expression in Escherichia coli | Bacillus subtilis |
1.1.1.85 | construction of a chimeric gene by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, expression in Escherichia coli | Thermus thermophilus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.85 | hanging drop vapor diffusion method, chimeric enzyme constructed by fusing the gene of Bacillus subtilis and Thermus thermophilus coding for 3-isopropylmalate dehydrogenase, expression in Escherichia coli | Bacillus subtilis |
1.1.1.85 | hanging drop vapor diffusion method, chimeric enzyme constructed by fusing the gene of Bacillus subtilis and Thermus thermophilus coding for 3-isopropylmalate dehydrogenase, expression in Escherichia coli | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.85 | Bacillus subtilis | - |
- |
- |
1.1.1.85 | Thermus thermophilus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.85 | (2R,3S)-3-isopropylmalate + NAD+ | - |
Bacillus subtilis | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
1.1.1.85 | (2R,3S)-3-isopropylmalate + NAD+ | - |
Thermus thermophilus | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.85 | NAD+ | - |
Bacillus subtilis | |
1.1.1.85 | NAD+ | - |
Thermus thermophilus |