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Literature summary extracted from
- Glycerol protection and purification of Bacillus subtilis glucose dehydrogenase (1983), J. Biol. Chem., 258, 12558-12565.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.47 | stability increases in the presence of glycerol | Bacillus subtilis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.47 | 4.2 | - |
2-deoxy-D-glucose | pH 6.5 | Bacillus subtilis |  |
| 1.1.1.47 | 4.8 | - |
beta-D-glucose | pH 6.5, Km -value decreases with decreasing pH | Bacillus subtilis | |
| 1.1.1.47 | 35 | - |
D-glucosamine | pH 6.5 | Bacillus subtilis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.47 | 31500 | - |
4 * 31500, SDS-PAGE | Bacillus subtilis |
| 1.1.1.47 | 126000 | - |
gel filtration | Bacillus subtilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.47 | Bacillus subtilis | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.47 | 368 | - |
- |
Bacillus subtilis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.47 | -20°C, 20% glycerol, 4 years, stable | Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.47 | 2-deoxy-D-glucose + NADP+ | - |
Bacillus subtilis | 2-deoxy-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
| 1.1.1.47 | beta-D-glucose + NAD+ | - |
Bacillus subtilis | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
| 1.1.1.47 | D-glucosamine + NAD+ | - |
Bacillus subtilis | D-glucosamino-1,5-lactone + NADH | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.47 | tetramer | 4 * 31500, SDS-PAGE | Bacillus subtilis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.47 | 6 | 6.5 | optimal stability | Bacillus subtilis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.47 | NAD+ | - |
Bacillus subtilis | |
| 1.1.1.47 | NADP+ | - |
Bacillus subtilis | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.1.1.47 | Bacillus subtilis | isoelectric focusing or chromatofocusing | 4.8 | 4.7 |
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