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Literature summary extracted from

  • Reguera, R.M.; Balana-Fouce, R.; Perez-Pertejo, Y.; Fernandez, F.J.; Garcia-Estrada, C.; Cubria, J.C.; Ordonez, C.; Ordonez, D.
    Cloning expression and characterization of methionine adenosyltransferase in Leishmania infantum promastigotes (2002), J. Biol. Chem., 277, 3158-3167.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.6 His6-tagged fusion protein expressed in Escherichia coli Leishmania infantum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.6 ATP
-
 Leishmania infantum
2.5.1.6 diphosphate inhibition for S-adenosylmethionine and L-methonine Leishmania infantum
2.5.1.6 S-adenosyl-L-ethionine
-
 Leishmania infantum
2.5.1.6 S-adenosyl-L-homocysteine
-
 Leishmania infantum
2.5.1.6 S-adenosylmethionine more than 50% inhibition at 1 mM Leishmania infantum
2.5.1.6 tripolyphosphate competitive with ATP; non competitive with L-methionine Leishmania infantum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.6 49000
-
 native MAT-II, gel filtration Leishmania infantum
2.5.1.6 249000
-
 recombinant MAT-II, gel filtration Leishmania infantum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.6 Leishmania infantum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.6 recombinant MAT II purified by a method that includes Ni-agarose bead affinity capture. Alternative method includes Triton X-100 and 8 mM urea buffered extraction of inclusion body phase and dialysis Leishmania infantum

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.6 ATP + L-methionine + H2O
-
 Leishmania infantum phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.6 dimer
-
 Leishmania infantum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.6 0.0000883
-
 ATP
-
 Leishmania infantum
2.5.1.6 0.0000883
-
 S-adenosylmethionine
-
 Leishmania infantum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.6 0.2
-
 tripolyphosphate inhibition with respect to L-methionine Leishmania infantum
2.5.1.6 0.25
-
 tripolyphosphate inhibition with respect to ATP Leishmania infantum
2.5.1.6 0.3
-
 diphosphate inhibition with respect to ATP Leishmania infantum
2.5.1.6 0.35
-
 diphosphate inhibition with respect to L-methionine Leishmania infantum
2.5.1.6 0.8
-
 ATP noncompetitive inhibition at 0.5-5 mM ATP concentrations Leishmania infantum
2.5.1.6 4
-
 S-adenosylmethionine noncompetitive inhibition at 0.5-5 mM L-methionine concentrations Leishmania infantum