EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.10 | additional information | not activated by Tween 20, Triton X-100, Chaps or Nonidet P-40 | Abies grandis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.10 | diphosphate | 0.66 mM, 50% inhibition | Abies grandis | |
2.5.1.10 | farnesyl diphosphate | 0.06 mM, 50% inhibition | Abies grandis | |
2.5.1.10 | K+ | 500 mM, 63% inhibition in the presence of MgCl2 | Abies grandis | |
2.5.1.10 | Mn2+ | strong inhibition above 0.1 mM, activation below | Abies grandis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.10 | 0.0018 | - |
geranyl diphosphate | - |
Abies grandis | |
2.5.1.10 | 0.009 | - |
dimethylallyl diphosphate | - |
Abies grandis | |
2.5.1.10 | 0.0153 | - |
isopentenyl diphosphate | - |
Abies grandis | |
2.5.1.10 | 0.0445 | - |
Mn2+ | - |
Abies grandis | |
2.5.1.10 | 0.0712 | - |
Mg2+ | - |
Abies grandis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.10 | Ca2+ | 18% of Mg2+ activation at 0.5 mM, inhibition above | Abies grandis | |
2.5.1.10 | Co2+ | 78% of Mg2+ activation at 1 mM | Abies grandis | |
2.5.1.10 | Mg2+ | maximal activity at 1 mM | Abies grandis | |
2.5.1.10 | Mg2+ | required for activity | Abies grandis | |
2.5.1.10 | Mn2+ | can replace Mg2+, maximal activity at 0.1 mM, inhibition above | Abies grandis | |
2.5.1.10 | Ni2+ | 10% of Mg2+ activation at 0.5 mM, inhibition above | Abies grandis | |
2.5.1.10 | Zn2+ | 91% of Mg2+ activation at 0.5 mM, almost complete inhibition at 5 mM | Abies grandis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.10 | 110000 | - |
gel filtration | Abies grandis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.10 | Abies grandis | - |
grand fir | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.10 | DEAE-Sepharose, Mono Q, phenyl-Sepharose | Abies grandis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.10 | sapling | - |
Abies grandis | - |
EC Number | Storage Stability | Organism |
---|---|---|
2.5.1.10 | -80°C, 3 months, no loss of activity | Abies grandis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.10 | dimethylallyl diphosphate + 2 isopentenyl diphosphate | - |
Abies grandis | (E,E)-farnesyl diphosphate + 2 diphosphate | - |
? | |
2.5.1.10 | geranyl diphosphate + isopentenyl diphosphate | - |
Abies grandis | diphosphate + (2E,6E)-farnesyl diphosphate | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.10 | 7.5 | 8 | - |
Abies grandis |