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Literature summary extracted from

  • Sinha, A.K.; Pathre, U.; Sane, P.V.
    Purification and characterization of sucrose-phosphate synthase from Prosopis juliflora (1997), Phytochemistry, 46, 441-447.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.14 glucose-6-phosphate
-
 Spinacia oleracea
2.4.1.14 glucose-6-phosphate
-
 Prosopis juliflora
2.4.1.14 Mg2+ stimulates enzyme activity Spinacia oleracea
2.4.1.14 Mg2+ stimulates enzyme activity Prosopis juliflora
2.4.1.14 Mn2+ stimulates enzyme activity Prosopis juliflora

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.14 ADP no inhibition Prosopis juliflora
2.4.1.14 ADP
-
 Spinacia oleracea
2.4.1.14 ATP reversible by addition of Mg2+ Prosopis juliflora
2.4.1.14 ATP
-
 Spinacia oleracea
2.4.1.14 CTP reversible by addition of Mg2+ Prosopis juliflora
2.4.1.14 fluoride strong inhibition Prosopis juliflora
2.4.1.14 fluoride
-
 Spinacia oleracea
2.4.1.14 GTP reversible by addition of Mg2+ Prosopis juliflora
2.4.1.14 ITP reversible by addition of Mg2+ Prosopis juliflora
2.4.1.14 p-chloromercuribenzoate activity can be restored by addition of DTT or 2-mercaptoethanol Prosopis juliflora
2.4.1.14 phosphate competitive with UDP-glucose Prosopis juliflora
2.4.1.14 phosphate competitive with UDP-glucose Spinacia oleracea
2.4.1.14 sulfate
-
 Prosopis juliflora
2.4.1.14 sulfate
-
 Spinacia oleracea
2.4.1.14 UDP competitive with UDP-glucose Prosopis juliflora
2.4.1.14 UDP
-
 Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.14 2
-
 D-fructose 6-phosphate Km is not effected by addition of 20 mM glucose 6-phosphate or 15 mM phosphate Prosopis juliflora
2.4.1.14 4.6
-
 UDP-glucose in presence of 20 mM glucose 6-phosphate Prosopis juliflora
2.4.1.14 20
-
 UDP-glucose
-
 Prosopis juliflora
2.4.1.14 77
-
 UDP-glucose in presence of 15 mM phosphate Prosopis juliflora

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.14 Mg2+ stimulates enzyme activity Spinacia oleracea
2.4.1.14 Mg2+ stimulates enzyme activity Prosopis juliflora
2.4.1.14 Mn2+
-
 Spinacia oleracea
2.4.1.14 Mn2+ stimulates enzyme activity Prosopis juliflora

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.14 443000
-
 gel filtration Prosopis juliflora

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate Spinacia oleracea catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
 ?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate Prosopis juliflora catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.14 Prosopis juliflora
-
-
-
2.4.1.14 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.14 partial, at least 3 additional proteins could not be removed Prosopis juliflora

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.14 leaf
-
 Spinacia oleracea
-
2.4.1.14 leaf
-
 Prosopis juliflora
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.14 28
-
 purified enzyme, specific activity in crude extract is extremely low compared to spinach Prosopis juliflora

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate
-
 Spinacia oleracea UDP + sucrose 6-phosphate
-
 ?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate ordered bi-bi mechanism Prosopis juliflora UDP + sucrose 6-phosphate
-
 ?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Spinacia oleracea UDP + sucrose 6-phosphate
-
 ?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Prosopis juliflora UDP + sucrose 6-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.14 25
-
-
 Prosopis juliflora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.14 7.5
-
-
 Prosopis juliflora