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Literature summary extracted from

  • Pharr, D.M.; Sox, H.N.; Locy, R.D.; Huber, S.C.
    Partial characterization of the galactinol forming enzyme from leaves of Cucumis sativus L. (1981), Plant Sci. Lett., 23, 25-33.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.4.1.123 70-80% loss of activity during purification Cucumis sativus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.123 additional information not in lysates of chloroplasts from leaves Cucumis sativus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.123 Mn2+ concentration influences pH-optimum Cucumis sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.123 UDP-galactose + myo-inositol Cucumis sativus responsible for galactinol synthesis, involved in raffinose and stachyose biosynthesis, cf. EC 2.4.1.67 and EC 2.4.1.82 UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.123 Cucumis sativus
-
 cucumber, cv. Chipper
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.123 partial, 41fold Cucumis sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.123 leaf 7th node from the growing tip, from fruiting plants Cucumis sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.123 additional information
-
-
 Cucumis sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.123 UDP-galactose + myo-inositol
-
 Cucumis sativus UDP + 1-O-alpha-D-galactosyl-D-myo-inositol identical with galactinol ?
2.4.1.123 UDP-galactose + myo-inositol responsible for galactinol synthesis, involved in raffinose and stachyose biosynthesis, cf. EC 2.4.1.67 and EC 2.4.1.82 Cucumis sativus UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.123 More cf. EC 2.4.1.67 and EC 2.4.1.82 Cucumis sativus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.123 30
-
 assay at Cucumis sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.123 additional information
-
 Mn2+-concentration influences pH-optimum Cucumis sativus
2.4.1.123 5.5
-
 at 7 mM Mn2+ Cucumis sativus
2.4.1.123 5.6
-
 assay at Cucumis sativus
2.4.1.123 6.2
-
 at 2 mM Mn2+ Cucumis sativus
2.4.1.123 7
-
 at 0.2 mM Mn2+, broad activity optimum centered around pH 7 Cucumis sativus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.123 5 7 about half-maximal activity at pH 5 and 7, at 7 mM Mn2+ Cucumis sativus
2.4.1.123 5.1 7.8 about half-maximal activity at pH 5.1 and 7.8, at 2.0 mM Mn2+ Cucumis sativus
2.4.1.123 5.2 8.6 about half-maximal activity at pH 5.2 and 8.6, at 0.2 mM Mn2+ Cucumis sativus