Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sasaki, K.; Kurata-Miura, K.; Ujita, M.; Angata, K.; Nakagawa, S.; Sekine, S.; Nishi, T.; Fukuda, M.
    Expression cloning of cDNA encoding a human beta-1,3-N-acetylglucosaminyltransferase that is essential for poly-N-acetyllactosamine synthesis (1997), Proc. Natl. Acad. Sci. USA, 94, 14294-14299.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.149 cDNA from melanoma and colonic cancer cells is cloned and overexpressed in human Burkitt lymphoma Namalwa KJM-1 cells overexpressing the i-antigen, nucleotide and amino acid sequence of the 415-amino acids protein, cDNA fragment encoding the stem region plus putative catalytic domain of iGnT fused to protein A is expressed in COS-1 cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.149 Golgi apparatus stem region and large catalytic domain reside presumably in the Golgi lumen Homo sapiens 5794
-
2.4.1.149 membrane type II membrane protein topology, longest transmembrane domain among glycosyltransferases cloned so far Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.149 47125
-
 x * 47125, calculated from the amino acid sequence, mature N-glycosylated iGnT: may be approximately 53 kDa Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.149 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R Homo sapiens enzyme is essential for the formation of poly-N-acetyllactosamines and the i-antigen, responsible for the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R structure and poly-N-acetyllactosamine extension UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.149 UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.149 Homo sapiens O43505
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.149 glycoprotein 2 potential N-glycosylation sites Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.149 brain highly expressed in fetal and adult brain Homo sapiens
-
2.4.1.149 kidney highly expressed in fetal kidney Homo sapiens
-
2.4.1.149 additional information enzyme is expressed ubiquitously in various adult tissues Homo sapiens
-
2.4.1.149 SW-1116 cell colonic carcinoma cell line Homo sapiens
-
2.4.1.149 WM-266-4 cell human melanoma cell line Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.149 additional information no acceptor: Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc Homo sapiens ?
-
 ?
2.4.1.149 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R iGnT has a unique characteristic in binding to acceptor substrates, preferentially adding poly-N-acetyllactosamine to membrane glycoproteins Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.149 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R enzyme is essential for the formation of poly-N-acetyllactosamines and the i-antigen, responsible for the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R structure and poly-N-acetyllactosamine extension Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.149 UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
 Homo sapiens UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
 ?
2.4.1.149 UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
-
 Homo sapiens ?
-
 ?
2.4.1.149 UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
-
 Homo sapiens UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.149 ? x * 47125, calculated from the amino acid sequence, mature N-glycosylated iGnT: may be approximately 53 kDa Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.149 37
-
 assay at Homo sapiens