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Literature summary extracted from

  • van den Eijnden, D.H.; Koenderman, A.H.L.; Schiphorst, W.E.C.M.
    Biosynthesis of blood group i-active polylactosaminoglycans. Partial purification and properties of an UDP-GlcNAc:N-acetyllactosaminide beta 1-->3-N-acetylglucosaminyltransferase from Novikoff tumor cell ascites fluid (1988), J. Biol. Chem., 263, 12461-12471.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.149 CaCl2 weak Rattus norvegicus
2.4.1.149 CdCl2
-
 Rattus norvegicus
2.4.1.149 EDTA strong Rattus norvegicus
2.4.1.149 FeCl2
-
 Rattus norvegicus
2.4.1.149 NiCl2
-
 Rattus norvegicus
2.4.1.149 ZnCl2 strong Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.149 additional information
-
 additional information
-
 Rattus norvegicus
2.4.1.149 0.6
-
 asialo-alpha1-acid glycoprotein
-
 Rattus norvegicus
2.4.1.149 1
-
 Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAc
-
 Rattus norvegicus
2.4.1.149 2.2
-
 N-acetyllactosamine
-
 Rattus norvegicus
2.4.1.149 5.2
-
 lactose
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.149 Mn2+ MnCl2 stimulates 2.5fold, optimal concentration: 20 mM Rattus norvegicus
2.4.1.149 additional information absolute requirement for divalent cations, not stimulated by Mg2+ Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.149 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R Rattus norvegicus enzyme functions in both the initiation and elongation of linear i-active polylactosaminoglycan chains of N-glycoproteins and possibly other glycoconjugates UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.149 UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine Rattus norvegicus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.149 Rattus norvegicus
-
 Sprague Dawley rats
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.149 glycoprotein
-
 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.149 partial, 178fold, from Novikoff tumor cell ascites fluid Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.149 Novikoff ascites tumor cell strain N1S1-67 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.149 0.00153
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.4.1.149 0-4°C, lyophilized enzyme form, over 6 months, stable Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.149 additional information asialo-serum glycoproteins are much more effective than O-glycoproteins as acceptors, overview over oligosaccharide substrates Rattus norvegicus ?
-
 ?
2.4.1.149 UDP-N-acetyl-alpha-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAc
-
 Rattus norvegicus UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAc
-
 ?
2.4.1.149 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R highly specific for acceptor oligosaccharides and glycoproteins carrying a terminal Galbeta(1-4)GlcNAcbeta1-R unit, catalyzes the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta-R sequence, Galbeta(1-4)GlcNAcbeta(1-2)(Galbeta(1-4)GlcNAcbeta(1-6))Man pentasaccharide in the acceptor structure is a requirement for optimal activity, branch specificity, branches of this pentasaccharide structure, when contained in tri- and tetraantennary oligosaccharides, are highly preferred over other branches for attachment of the 1st and 2nd mol of GlcNAc into the acceptor molecule, enzyme also shows activity towards oligosaccharides related to blood group I- and i-active polylactosaminoglycans Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.149 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R enzyme functions in both the initiation and elongation of linear i-active polylactosaminoglycan chains of N-glycoproteins and possibly other glycoconjugates Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.149 UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
-
 Rattus norvegicus ?
-
 ?
2.4.1.149 UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein much more effective than asialo-transferrin and asialo-fetuin, asialo-alpha1-acid glycoprotein from human plasma Cohn fraction V, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage Rattus norvegicus UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
 ?
2.4.1.149 UDP-N-acetylglucosamine + asialo-fetuin much less effective than asialo-alpha1-acid glycoprotein, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage Rattus norvegicus UDP + N-acetylglucosaminylated asialo-fetuin
-
 ?
2.4.1.149 UDP-N-acetylglucosamine + asialo-transferrin much less effective than asialo-alpha1-acid glycoprotein, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage Rattus norvegicus UDP + N-acetylglucosaminylated asialo-transferrin
-
 ?
2.4.1.149 UDP-N-acetylglucosamine + lactose 70% of activity with N-acetyllactosamine Rattus norvegicus UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
 ?
2.4.1.149 UDP-N-acetylglucosamine + N-acetyllactosamine more effective acceptor than lactose Rattus norvegicus UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
 ?
2.4.1.149 UDP-N-acetylglucosamine + porcine submaxillary asialo-afuco-mucin very poor substrate Rattus norvegicus UDP + N-acetylglucosaminylated porcine submaxillary asialo-afuco-mucin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.149 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.149 6.8 7.2 asialo-alpha1-acid glycoprotein as acceptor Rattus norvegicus