Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Liu, M.; Cao, D.; Russell, R.; Handschumacher, R.E.; Pizzorno, G.
    Expression, characterization, and detection of human uridine phosphorylase and identification of variant uridine phosphorolytic activity in selected human tumors (1998), Cancer Res., 58, 5418-5424.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.3 fusion protein with maltose-binding protein Homo sapiens
2.4.2.4
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.3 5-benzylacyclouridine 0.01 mM, 90% inhibition of normal tissues, 40-60% inhibition of carcinoma tissue Homo sapiens
2.4.2.4 5-benzylacyclouridine
-
 Homo sapiens
2.4.2.4 5-bromo-6-aminouracil
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.3 0.076
-
 ribose 1-phosphate
-
 Homo sapiens
2.4.2.3 0.189
-
 uridine
-
 Homo sapiens
2.4.2.3 0.202
-
 phosphate
-
 Homo sapiens
2.4.2.3 0.209
-
 Uracil
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.3 32000 35000 SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.4 thymine + 2-deoxy-D-ribose 1-phosphate Homo sapiens degradation of uridine in tumor specimen thymidine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.3 Homo sapiens
-
-
-
2.4.2.3 Mus musculus
-
-
-
2.4.2.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.3
-
 Mus musculus
2.4.2.3
-
 Homo sapiens
2.4.2.4 separation of uridine phosphorylase and thymidine phosphorylase activities Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.3 colorectal adenocarcinoma cell colon 26 tumor cells transplanted in BALB/c mice Mus musculus
-
2.4.2.4 breast tumor specimen Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.3 89.6
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.3 2'-deoxyuridine + phosphate
-
 Mus musculus uracil + deoxyribose 1-phosphate
-
 ?
2.4.2.3 2'-deoxyuridine + phosphate
-
 Homo sapiens uracil + deoxyribose 1-phosphate
-
 ?
2.4.2.3 thymidine + phosphate
-
 Mus musculus thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 thymidine + phosphate
-
 Homo sapiens thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 uridine + phosphate
-
 Mus musculus uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.3 uridine + phosphate
-
 Homo sapiens uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.4 thymine + 2-deoxy-D-ribose 1-phosphate degradation of uridine in tumor specimen Homo sapiens thymidine + phosphate
-
 ?
2.4.2.4 uridine + phosphate
-
 Homo sapiens uracil + ribose 1-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.3 7.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.3 7 7.75 pH 6.5: about 70% of activity maximum, pH 8.5: about 25% of activity maximum Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.3 0.0073
-
 5-benzylacyclouridine
-
 Homo sapiens