BRENDA - Enzyme Database

Purification and characterization of L-mimosine synthase from Leucaema leucocephala

Murakoshi, I.; Ikegami, F.; Hinuma, Y.; Hanma, Y.; Phytochemistry 23, 1905-1908 (1984)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.52
5
-
3,4-dihydroxypyridine
-
Leucaena leucocephala
2.5.1.52
6.25
-
O-acetylserine
-
Leucaena leucocephala
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.52
32000
-
2 * 32000, SDS-PAGE
Leucaena leucocephala
2.5.1.52
64000
-
gel filtration
Leucaena leucocephala
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.5.1.52
O3-acetyl-L-serine + 3,4-dihydroxypyridine
Leucaena leucocephala
involved in biosynthesis of heterocyclic beta-substituted alanines, brings about the biosynthesis of L-mimosine
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.5.1.52
Leucaena leucocephala
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.5.1.52
partial, to near homogeneity
Leucaena leucocephala
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.5.1.52
seedling
cotyledons removed
Leucaena leucocephala
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.5.1.52
0.189
-
-
Leucaena leucocephala
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.52
additional information
no activity with O-phospho-L-serine and L-Ser
638434
Leucaena leucocephala
?
-
-
-
-
2.5.1.52
O-succinyl-L-serine + 3,4-dihydroxypyridine
60% of the activity with O-acetylserine
638434
Leucaena leucocephala
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + succinate
-
-
-
?
2.5.1.52
O-sulfo-L-serine + 3,4-dihydroxypyridine
32% of the activity with O-acetyl-L-serine
638434
Leucaena leucocephala
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + sulfate
-
-
-
?
2.5.1.52
O3-acetyl-L-serine + 3,4-dihydroxypyridine
best substrates
638434
Leucaena leucocephala
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
i.e. L-mimosine
638434
Leucaena leucocephala
?
2.5.1.52
O3-acetyl-L-serine + 3,4-dihydroxypyridine
involved in biosynthesis of heterocyclic beta-substituted alanines, brings about the biosynthesis of L-mimosine
638434
Leucaena leucocephala
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
-
-
-
-
2.5.1.52
O3-acetyl-L-serine + 3-amino-1,2,4-triazole
30% of the activity with 3,4-dihydroxypyridine
638434
Leucaena leucocephala
3-(3-amino-1,2,4-triazol-1-yl)-L-alanine + acetate
-
638434
Leucaena leucocephala
?
2.5.1.52
O3-acetyl-L-serine + N-hydroxyurea
13% of the activity with 3,4-dihydroxypyridine
638434
Leucaena leucocephala
ureidoserine + acetate
-
638434
Leucaena leucocephala
?
2.5.1.52
O3-acetyl-L-serine + pyrazole
82% of the activity with 3,4-dihydroxypyridine
638434
Leucaena leucocephala
3-(pyrazol-1-yl)-L-alanine + acetate
-
638434
Leucaena leucocephala
?
Subunits
EC Number
Subunits
Commentary
Organism
2.5.1.52
dimer
2 * 32000, SDS-PAGE
Leucaena leucocephala
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.52
7.8
-
reaction with 3,4-dihydroxypyridine
Leucaena leucocephala
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.5.1.52
pyridoxal 5'-phosphate
requirement, enzyme bound, 1 mol pyridoxal 5'-phosphate per mol subunit
Leucaena leucocephala
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.5.1.52
pyridoxal 5'-phosphate
requirement, enzyme bound, 1 mol pyridoxal 5'-phosphate per mol subunit
Leucaena leucocephala
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.52
5
-
3,4-dihydroxypyridine
-
Leucaena leucocephala
2.5.1.52
6.25
-
O-acetylserine
-
Leucaena leucocephala
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.52
32000
-
2 * 32000, SDS-PAGE
Leucaena leucocephala
2.5.1.52
64000
-
gel filtration
Leucaena leucocephala
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.5.1.52
O3-acetyl-L-serine + 3,4-dihydroxypyridine
Leucaena leucocephala
involved in biosynthesis of heterocyclic beta-substituted alanines, brings about the biosynthesis of L-mimosine
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.52
partial, to near homogeneity
Leucaena leucocephala
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.5.1.52
seedling
cotyledons removed
Leucaena leucocephala
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.5.1.52
0.189
-
-
Leucaena leucocephala
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.52
additional information
no activity with O-phospho-L-serine and L-Ser
638434
Leucaena leucocephala
?
-
-
-
-
2.5.1.52
O-succinyl-L-serine + 3,4-dihydroxypyridine
60% of the activity with O-acetylserine
638434
Leucaena leucocephala
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + succinate
-
-
-
?
2.5.1.52
O-sulfo-L-serine + 3,4-dihydroxypyridine
32% of the activity with O-acetyl-L-serine
638434
Leucaena leucocephala
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + sulfate
-
-
-
?
2.5.1.52
O3-acetyl-L-serine + 3,4-dihydroxypyridine
best substrates
638434
Leucaena leucocephala
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
i.e. L-mimosine
638434
Leucaena leucocephala
?
2.5.1.52
O3-acetyl-L-serine + 3,4-dihydroxypyridine
involved in biosynthesis of heterocyclic beta-substituted alanines, brings about the biosynthesis of L-mimosine
638434
Leucaena leucocephala
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
-
-
-
-
2.5.1.52
O3-acetyl-L-serine + 3-amino-1,2,4-triazole
30% of the activity with 3,4-dihydroxypyridine
638434
Leucaena leucocephala
3-(3-amino-1,2,4-triazol-1-yl)-L-alanine + acetate
-
638434
Leucaena leucocephala
?
2.5.1.52
O3-acetyl-L-serine + N-hydroxyurea
13% of the activity with 3,4-dihydroxypyridine
638434
Leucaena leucocephala
ureidoserine + acetate
-
638434
Leucaena leucocephala
?
2.5.1.52
O3-acetyl-L-serine + pyrazole
82% of the activity with 3,4-dihydroxypyridine
638434
Leucaena leucocephala
3-(pyrazol-1-yl)-L-alanine + acetate
-
638434
Leucaena leucocephala
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.5.1.52
dimer
2 * 32000, SDS-PAGE
Leucaena leucocephala
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.52
7.8
-
reaction with 3,4-dihydroxypyridine
Leucaena leucocephala