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Literature summary extracted from

  • Murakoshi, I.; Ikegami, F.; Hinuma, Y.; Hanma, Y.
    Purification and characterization of L-mimosine synthase from Leucaema leucocephala (1984), Phytochemistry, 23, 1905-1908.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.52 5
-
 3,4-dihydroxypyridine
-
 Leucaena leucocephala
2.5.1.52 6.25
-
 O-acetylserine
-
 Leucaena leucocephala

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.52 32000
-
 2 * 32000, SDS-PAGE Leucaena leucocephala
2.5.1.52 64000
-
 gel filtration Leucaena leucocephala

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.52 O3-acetyl-L-serine + 3,4-dihydroxypyridine Leucaena leucocephala involved in biosynthesis of heterocyclic beta-substituted alanines, brings about the biosynthesis of L-mimosine 3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.52 Leucaena leucocephala
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.52 partial, to near homogeneity Leucaena leucocephala

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.52 seedling cotyledons removed Leucaena leucocephala
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.52 0.189
-
-
 Leucaena leucocephala

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.52 additional information no activity with O-phospho-L-serine and L-Ser Leucaena leucocephala ?
-
 ?
2.5.1.52 O-succinyl-L-serine + 3,4-dihydroxypyridine 60% of the activity with O-acetylserine Leucaena leucocephala 3-(3,4-dihydroxypyridin-1-yl)-L-alanine + succinate
-
 ?
2.5.1.52 O-sulfo-L-serine + 3,4-dihydroxypyridine 32% of the activity with O-acetyl-L-serine Leucaena leucocephala 3-(3,4-dihydroxypyridin-1-yl)-L-alanine + sulfate
-
 ?
2.5.1.52 O3-acetyl-L-serine + 3,4-dihydroxypyridine best substrates Leucaena leucocephala 3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate i.e. L-mimosine ?
2.5.1.52 O3-acetyl-L-serine + 3,4-dihydroxypyridine involved in biosynthesis of heterocyclic beta-substituted alanines, brings about the biosynthesis of L-mimosine Leucaena leucocephala 3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
-
 ?
2.5.1.52 O3-acetyl-L-serine + 3-amino-1,2,4-triazole 30% of the activity with 3,4-dihydroxypyridine Leucaena leucocephala 3-(3-amino-1,2,4-triazol-1-yl)-L-alanine + acetate
-
 ?
2.5.1.52 O3-acetyl-L-serine + N-hydroxyurea 13% of the activity with 3,4-dihydroxypyridine Leucaena leucocephala ureidoserine + acetate
-
 ?
2.5.1.52 O3-acetyl-L-serine + pyrazole 82% of the activity with 3,4-dihydroxypyridine Leucaena leucocephala 3-(pyrazol-1-yl)-L-alanine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.52 dimer 2 * 32000, SDS-PAGE Leucaena leucocephala

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.52 7.8
-
 reaction with 3,4-dihydroxypyridine Leucaena leucocephala

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.52 pyridoxal 5'-phosphate requirement, enzyme bound, 1 mol pyridoxal 5'-phosphate per mol subunit Leucaena leucocephala