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Literature summary extracted from

  • Aldritt, S.M.; Wang, C.C.
    Purification and characterization of guanine phosphoribosyltransferase from Giardia lamblia (1986), J. Biol. Chem., 261, 8528-8533.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.8 29000
-
 2 * 29000, SDS-PAGE Giardia intestinalis
2.4.2.8 58000 63000 gel filtration, isokinetic sucrose density gradient centrifugation Giardia intestinalis

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Giardia intestinalis
-
 3 charge variant forms
-
2.4.2.8 Giardia intestinalis Portland
-
 3 charge variant forms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.8
-
 Giardia intestinalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.8 trophozoite
-
 Giardia intestinalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.8 additional information
-
-
 Giardia intestinalis

Storage Stability

EC Number Storage Stability Organism
2.4.2.8 -80°C Giardia intestinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Giardia intestinalis GMP + diphosphate
-
 ?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Giardia intestinalis Portland GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Giardia intestinalis IMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Giardia intestinalis Portland IMP + diphosphate
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Giardia intestinalis XMP + diphosphate
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Giardia intestinalis Portland XMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.8 dimer 2 * 29000, SDS-PAGE Giardia intestinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.8 additional information
-
 3 charge variant forms: pIs of 6.75, 5.3, 5.2 Giardia intestinalis
2.4.2.8 7 9 broad maximum Giardia intestinalis